Anti-Adenosine A1 Receptor antibody (ab82477)

Overview

  • Product name
    Anti-Adenosine A1 Receptor antibody
    See all Adenosine A1 Receptor primary antibodies
  • Description
    Rabbit polyclonal to Adenosine A1 Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Rat Adenosine A1 Receptor.

    (Peptide available as ab91076.)

  • Positive control
    • This antibody gave a positive signal in Rat Spinal Ganglion Neuronal cell lysate and Mouse EL4 Lymphoma whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • All lanes : Anti-Adenosine A1 Receptor antibody (ab82477) at 1 µg/ml

    Lane 1 : Rat Spinal Ganglion Neuronal cell lysate
    Lane 2 : EL4 whole cell lysate (ab7183)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 118 kDa, 18 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • ICC/IF image of ab82477 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82477, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
  • IHC image of ab82477 staining in Rat Adult Brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82477, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Navarro G  et al. Cross-communication between Gi and Gs in a G-protein-coupled receptor heterotetramer guided by a receptor C-terminal domain. BMC Biol 16:24 (2018). Read more (PubMed: 29486745) »
  • Zhai W  et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). IF ; Rat . Read more (PubMed: 27301321) »

See all 9 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Sample
Human Tissue sections (Brain cortex)
Specification
Brain cortex
Permeabilization
Yes - TBS-T (0.05% tween)
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20% or 10%)
Sample
Human Cell lysate - whole cell (CHO-cells)
Specification
CHO-cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. David Wilkes

Verified customer

Submitted May 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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