Anti-Adenosine A1 Receptor antibody [EPR6179] (ab124780)

Overview

  • Product nameAnti-Adenosine A1 Receptor antibody [EPR6179]
    See all Adenosine A1 Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR6179] to Adenosine A1 Receptor
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the extracellular domain of Adenosine A1 Receptor (Human)

  • Positive control
    • WB: Saos 2, SH SY5Y, Caco 2, A549 or 293T cell lysates IHC-P: Human breast tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/100.

Target

  • FunctionReceptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1AR antibody
    • A1R antibody
    • AA1R antibody
    • AA1R_HUMAN antibody
    • Adenosine receptor A1 antibody
    • ADORA 1 antibody
    • ADORA 1 antibody
    • ADORA1 antibody
    • RDC 7 antibody
    • RDC7 antibody
    • Ri antibody
    see all

Anti-Adenosine A1 Receptor antibody [EPR6179] images

  • Overlay histogram showing SH-SY5Y cells stained with ab124780 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124780, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab124780, at 1/100, staining Adenosine A1 Receptor in formalin fixed paraffin embedded Human breast tissue using immunohistochemistry.
  • All lanes : Anti-Adenosine A1 Receptor antibody [EPR6179] (ab124780) at 1/1000 dilution

    Lane 1 : Saos 2 cell lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : Caco 2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled Goat anti Rabbit at 1/2000 dilution

    Predicted band size : 37 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-Adenosine A1 Receptor antibody [EPR6179] (ab124780)

ab124780 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
I am sorry to hear that the 2 antibodies do not seem to work as expected.

As we discussed over the phone, ab82477 has not been tested in human and chinese hamster samples. But if you could try it on mouse or ra...

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Following up my previous message, the immunogen for ab124780, an alternative to ab3460, is also unfortunately unlikely to react with canine Adenosine A1 Receptor. Alignment of the canine receptor amino acid sequence with that of the ab124780 immunogen ...

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Thank you so much for your reply.

I look forward to hear back from you and wish you good luck with ab124780. This antibodyhas been tested in WB with human samples, and thus, will be guaranteed.

I now have some additional information to share with you in regards to the antibodies I discussed in my last email against adenosine receptors.

Anti-Adenosine Receptor A2a

Unfortunately I can confirm that the other two antibodies I ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"