Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714)
Key features and details
- Mouse monoclonal [7F6-G5-A2] to Adenosine Receptor A2a
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]
See all Adenosine Receptor A2a primary antibodies -
Description
Mouse monoclonal [7F6-G5-A2] to Adenosine Receptor A2a -
Host species
Mouse -
Specificity
This antibody is recommended for tissue lysates only. In house testing has shown no signal in Western Blot for SH-SY5Y, SK-N-SH, PC-12 or HeLa cell lines.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Hamster, Dog, Non human primates -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Epitope
Recognises amino acids 213-220 (SQPLPGER) within the third intracellular loop. -
Positive control
- WB: Human, mouse, and rat brain tissue lysates. IHC-P: Rat, mouse and human brain caudate nucleus.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified by running the antiserum from the injected animal through an affinity column with the antigen bound to a beaded agarose gel. -
Clonality
Monoclonal -
Clone number
7F6-G5-A2 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
We recommend using 1% BSA as a blocking agent for western blot. |
Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). We recommend using 1% BSA as a blocking agent for western blot. |
Target
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Function
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Domain
The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface. -
Post-translational
modificationsUbiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 135 Human
- Entrez Gene: 11540 Mouse
- Entrez Gene: 25369 Rat
- Omim: 102776 Human
- SwissProt: P11617 Dog
- SwissProt: P29274 Human
- SwissProt: Q60613 Mouse
- SwissProt: P30543 Rat
see all -
Alternative names
- A2AAR antibody
- A2aR antibody
- AA2AR_HUMAN antibody
see all
Images
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IHC image of Adenosine Receptor A2a staining in Mouse normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Lanes 1-3 : Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) at 5 µg/ml
Lanes 4-6 : Competitor product at 5 µg/ml
Lanes 1 & 4 : Human brain tissue lysate
Lanes 2 & 5 : Mouse brain tissue lysate
Lanes 3 & 6 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Additional bands at: 25 kDa, 39 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesBlocking buffer: 1% BSA
Gel type: MOPS
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IHC image of Adenosine Receptor A2a staining in Human normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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IHC image of Adenosine Receptor A2a staining in Rat normal brain Caudate Nucleus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79714, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab79714 has been referenced in 6 publications.
- Stocco E et al. Experimental Evidence of A2A-D2 Receptor-Receptor Interactions in the Rat and Human Carotid Body. Front Physiol 12:645723 (2021). PubMed: 33935801
- Sánchez-Melgar A et al. Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol. Cells 9:N/A (2020). PubMed: 32645849
- Huang W et al. An adenosine A1R-A2aR imbalance regulates low glucose/hypoxia-induced microglial activation, thereby contributing to oligodendrocyte damage through NF-?B and CREB phosphorylation. Int J Mol Med 41:3559-3569 (2018). PubMed: 29512780
- Sakano D et al. Dopamine D2 Receptor-Mediated Regulation of Pancreatic ß Cell Mass. Stem Cell Reports 7:95-109 (2016). Mouse . PubMed: 27373926
- Niesman IR et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation 11:39 (2014). WB ; Mouse . PubMed: 24593993
- Bone DB et al. Nucleoside/nucleobase transport and metabolism by microvascular endothelial cells isolated from ENT1-/- mice. Am J Physiol Heart Circ Physiol 299:H847-56 (2010). WB ; Mouse . PubMed: 20543083