Reacts with adenovirus serotypes 1 to 7, 40 and 41 (as primary isolates in tissue culture).
It does not crossreact with tissue culture isolates of respiratory syncytial virus, influenza virus types A and B, parainfluenza virus types 1, 2, 3 and 4b, herpes simplex virus types 1 and 2, varicella-zoster virus, cytomegalovirus, mumps virus, measles virus, ECHOvirus 19, coxsackie B4 virus, poliovirus types 1, 2 and 3 or negative tissue culture cells used in routine virus isolation.
Adenovirus type 3, strain GB (ATCC). Soluble antigen prepared by trichlorofluorethane treatment of infected cells.
Acetone-fixed HEp 2 cells infected with adenovirus type 2.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/10. The use of a fluorescence enhancing mounting medium is not recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.