Overview

  • Product nameAnti-Adenovirus antibody [B025 / AD51]
    See all Adenovirus primary antibodies
  • Description
    Mouse monoclonal [B025 / AD51] to Adenovirus
  • SpecificityThis antibody recognises all Adenovirus serotypes; binding to the hexon polypeptide. No cross-reactions are known.
  • Tested applicationsSuitable for: IHC-P, ELISA, IHC-Frmore details
  • Immunogen

    Adenovirus type 3 (ATCC strain VR847)

  • General notesWith recent studies indicating that viral agents may be implicated in new emerging diseases reported around the world, interest in viruses has increased and encompassed many different viral genera. The adenoviruses are represented by several different groups yet there are only few monoclonal antibodies that are commercially available that recognise all known serotypes.


    Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS with 0.1% sodium azide
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  • Primary antibody notesAdenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.
  • ClonalityMonoclonal
  • Clone numberB025 / AD51
  • MyelomaJK.Ag8.653
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. This product requires protein digestion pre-treatment of paraffin sections (e.g. trypsin or pronase) for 15 min prior to primary incubation
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceAdenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.
  • Alternative names
    • Adenovirus antibody

References for Anti-Adenovirus antibody [B025 / AD51] (ab7428)

This product has been referenced in:
  • Marschall M  et al. Assessment of drug candidates for broad-spectrum antiviral therapy targeting cellular pyrimidine biosynthesis. Antiviral Res 100:640-8 (2013). Read more (PubMed: 24149002) »
  • De Jong JC  et al. Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively. J Clin Microbiol 37:3940-5 (1999). Read more (PubMed: 10565911) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry regarding ab7428 (Mouse Anti-Adenovirus).
Indeed, this antibody does require pretreatment with a protease enzyme. The successful use of ab7428 in IHC-paraffin has been reported in a customer publication which used this s...

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I am glad finally the product gave you expected results.

Many thanks for your cooperation in this case.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for your email.

I am sorry to hear that the antibody did not work. The proteinase kInase K was the recommended antigen retrieval for this product so I am sorry there is nothing more we can suggest.

I can certainly send you a...

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Thank you for your enquiry.

I am sorry to confirm that we do not provide expiry dates. Antibodies should be stable for at least 1 year if stored correctly, as described on the individual datasheets.

I would like to reassure you th...

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Thank you very much for providing details.

I have few more question to understand the problem better:

- Is this ab fails to stain at all or showed high background?
- Have you tested the positivity of lung section by any other meth...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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Thank you for contacting us.

The information we have on the datasheet is from different publications; we unfortunately as such do not hold any tonsil related data. I apologize for being not helpful due to lack of data. I can recommend searchin...

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Thank you for contacting us and reporting your results with using Anti-Adenovirus antibody [B025 / AD51] ab7428 in ELISA.
I have had a look into the data we have for this antibody. Unfortunately, no epitope mapping has been performed with this ant...

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Thank you for your enquiry. Unfortunately I do not have details of the degree of reactivity of ab8249 and ab8251 against the Adenovirus subclasses. However, I can tell you that ab7428 recognises all Adenovirus serotypes. All antibodies are yet to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"