Overview

  • Product nameAnti-Adenovirus antibody [M58 + M73]See all Adenovirus primary antibodies ...
  • Description
    Mouse monoclonal [M58 + M73] to Adenovirus
  • Tested applicationsIHC-P, IHC-Fr, ICC/IF more details
  • Immunogen

    Adenovirus.

  • Positive control
    • Suitable tissue control sections for Immunohistochemistry: Adenovirus infected tissue.
  • General notes


    The adenovirus early gene products E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth inhibitory effects of TGF beta. The E1A region encodes a series of related proteins (35 - 46 kD) with multifunctional capabilities and forms a specific complex with the retinoblastoma tumor suppressor gene product. The E1a and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notes The adenovirus early gene products E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth inhibitory effects of TGF beta. The E1A region encodes a series of related proteins (35 - 46 kD) with multifunctional capabilities and forms a specific complex with the retinoblastoma tumor suppressor gene product. The E1a and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region.
  • Clonality Monoclonal
  • Clone numberM58 + M73
  • Myelomaunknown
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab3648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/50 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/50 - 1/75. ABC method.
ICC/IF Use at an assay dependent dilution. PubMed: 18287244

Target

  • RelevanceAdenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.
  • Alternative names
    • Adenovirus antibody

Anti-Adenovirus antibody [M58 + M73] images

  • ab3648 staining human infected appendix by IHC-P.

References for Anti-Adenovirus antibody [M58 + M73] (ab3648)

This product has been referenced in:
  • Magnusson MK  et al. A transductionally re-targeted adenovirus vector for virotherapy of Her2/neu expressing prostate cancer. Hum Gene Ther : (2011). IHC-P . Read more (PubMed: 21875358) »
  • McSharry BP  et al. Adenovirus E3/19K promotes evasion of NK cell recognition by intracellular sequestration of the NKG2D ligands major histocompatibility complex class I chain-related proteins A and B. J Virol 82:4585-94 (2008). ICC/IF . Read more (PubMed: 18287244) »

See all 3 Publications for this product

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ab3648 recognizes adenovirus serogroups 2 and 5.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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I am glad finally the product gave you expected results.

Many thanks for your cooperation in this case.

Thank you for your enquiry and your interest in our products. Epitope mappig experiments have not been carried out yet, so unfortunately we do not have any information about the epitope this antibody recognizes. We will update the on-line datas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"