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The adenovirus early gene products E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth inhibitory effects of TGF beta. The E1A region encodes a series of related proteins (35 - 46 kD) with multifunctional capabilities and forms a specific complex with the retinoblastoma tumor suppressor gene product. The E1a and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region.
Our Abpromise guarantee covers the use of ab3648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.|
|IHC-Fr||1/50 - 1/75. ABC method.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 18287244|
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