The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa.
Use at 2 µg/mg of lysate.
Native verified. Use Protein A.
Use at an assay dependent concentration.
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
RelevanceAdenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.
Formalin-fixed, paraffin-embedded human infected appendix stained with ab76550 using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of infected epithelial cells.
This image was generated using a version of this antibody containing BSA and sodium azide.
Western blot - Adenovirus antibody [M58 + M73] - BSA and Azide free (ab76550)
Anti-Adenovirus antibody [M58 + M73] (ab76550) at 2 µg/ml + Human cells 1