Anti-Adenovirus antibody [M58 + M73] (ab76550)

Overview

  • Product name
    Anti-Adenovirus antibody [M58 + M73]
    See all Adenovirus primary antibodies
  • Description
    Mouse monoclonal [M58 + M73] to Adenovirus
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Adenovirus
  • Immunogen

    Adenovirus

  • Positive control
    • 293 cells or adenovirus infected tissues
  • General notes

    This antibody detects adenovirus serogroups 2 and 5.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab7423 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    This antibody detects adenovirus serogroups 2 and 5.
  • Clonality
    Monoclonal
  • Clone number
    M58 + M73
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa.
IP Use at 2 µg/mg of lysate.

Native verified. Use Protein A.

ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Target

  • Relevance
    Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.
  • Alternative names
    • Adenovirus antibody

Images

  • Formalin-fixed, paraffin-embedded human infected appendix stained with ab76550 using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of infected epithelial cells. This image was generated using a version of this antibody containing BSA and sodium azide.
  • Anti-Adenovirus antibody [M58 + M73] (ab76550) at 2 µg/ml + Human cells 1

    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

References

ab76550 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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