Anti-Adenovirus antibody [M58 + M73] (ab76550)

Overview

  • Product nameAnti-Adenovirus antibody [M58 + M73]
    See all Adenovirus primary antibodies
  • Description
    Mouse monoclonal [M58 + M73] to Adenovirus
  • Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Adenovirus
  • Immunogen

    Adenovirus

  • Positive control
    • 293 cells or adenovirus infected tissues
  • General notes


    This antibody detects adenovirus serogroups 2 and 5.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThis antibody detects adenovirus serogroups 2 and 5.
  • ClonalityMonoclonal
  • Clone numberM58 + M73
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa.
IP Use at 2 µg/mg of lysate.

Native verified. Use Protein A.

ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Target

  • RelevanceAdenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.
  • Alternative names
    • Adenovirus antibody

Anti-Adenovirus antibody [M58 + M73] images

  • Formalin-fixed, paraffin-embedded human infected appendix stained with ab76550 using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of infected epithelial cells. This image was generated using a version of this antibody containing BSA and sodium azide.
  • Anti-Adenovirus antibody [M58 + M73] (ab76550) at 2 µg/ml + Human cells 1

    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

References for Anti-Adenovirus antibody [M58 + M73] (ab76550)

ab76550 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab76550.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"