Overview

  • Product nameAnti-Adenovirus E1A antibody [0.N.7]
    See all Adenovirus E1A primary antibodies
  • Description
    Mouse monoclonal [0.N.7] to Adenovirus E1A
  • SpecificityThis antibody is recommended for detection of E1A antigens of Adenovirus-2 and Adenovirus-5 origin.
  • Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IFmore details
  • Immunogen

    Adenovirus.

Properties

Applications

Our Abpromise guarantee covers the use of ab33183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml.
IP Use a concentration of 2 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceThe early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.
  • Cellular localizationNuclear
  • Alternative names
    • Control protein E1A antibody
    • E1A antibody

Anti-Adenovirus E1A antibody [0.N.7] images

  • Adenovirus infected Human appendix stained with ab33183 and DAB Chromagen.
  • 20ug cell lysate (cell line derived from Human Renal Cancer-2), primary antibody dilution is 1ug/ml, overnight incubation at 4°C.

References for Anti-Adenovirus E1A antibody [0.N.7] (ab33183)

This product has been referenced in:
  • Li G  et al. CD123 targeting oncolytic adenoviruses suppress acute myeloid leukemia cell proliferation in vitro and in vivo. Blood Cancer J 4:e194 (2014). WB . Read more (PubMed: 24658372) »
  • Gilbert R  et al. Establishment and validation of new complementing cells for production of E1-deleted adenovirus vectors in serum-free suspension culture. J Virol Methods 208:177-88 (2014). Read more (PubMed: 25159033) »

See all 5 Publications for this product

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Please find the Certificate of Compliance (CoC) for the lot GR56563-1 of ab33183 in the attachment.

The Material Safety Datasheet (MSDS) can be downloaded directly from the online datasheet through the li...

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Thank you for your enquiry. This antibody recognizes three adenovirus (Ad) 2 and 5 E1A gene products, with bands in the range 36-50kD. Different Ad2 infected cell lines can display one or more multiple bands. The Western Blot image provided us...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"