SpecificityThis antibody reacts with Adenovirus-infected cells and tissue. Using Immunoprecipitation it also recognizes adenovirus 2 and 5 E1A proteins, 3 bands at 30-50kDa, as well as, E1A associated proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml.
Use at an assay dependent concentration.
RelevanceThe early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.
Control protein E1A antibody
References for Anti-Adenovirus E1A antibody [M73] (ab28305)