Anti-Adenovirus hexon protein antibody [1E11] (ab8251)

Overview

  • Product name
    Anti-Adenovirus hexon protein antibody [1E11]
    See all Adenovirus hexon protein primary antibodies
  • Description
    Mouse monoclonal [1E11] to Adenovirus hexon protein
  • Specificity
    The antibody cross reacts with serotypes 1, 5, 7, 8, 12, 27 and 42. Isotype is a mixture of IgG2a and IgM.
  • Tested applications
    Suitable for: IHC-P, Sandwich ELISA, ELISA, Immunodiffusionmore details
  • Immunogen

    Hexon antigen of human and canine adenovirus (type 1)

  • General notes
    This antibody clone is biclonal, consisting of IgG2a and IgM isotypes.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    1E11
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Sandwich ELISA
ELISA
Immunodiffusion
  • Application notes
    ELISA: Use at an assay dependent concentration.
    ID: Use at an assay dependent concentration.
    sELISA: Use at an assay dependent dilution. Recommended for use as an ELISA pair with ab8249 (8C4)
  • Target

    • Relevance
      Hexon protein is a major coat protein of adenoviruses. Adenoviruses capsids have three principal protein components: the hexon, the penton, and the fiber. Hexon consists of three subunits together forming two major components of different morphological symmetry. A triangular top with three towers of density is superimposed on a more bulky pseudo hexagonal base. The symmetry of the top is in accord with the trimeric nature of hexon, but that of the base derives from the molecular function, which is to provide a densely packed impenetrable protective outer layer for the virion.
    • Cellular localization
      Major coat protein of adenoviruses.
    • Alternative names
      • Hexon protein antibody
      • Late protein 2 antibody
      • PII antibody

    References

    This product has been referenced in:
    • Yilmazer A  et al. Development of dual-activity vectors by co-envelopment of adenovirus and SiRNA in artificial lipid bilayers. PLoS One 9:e114985 (2014). Read more (PubMed: 25501573) »
    • Van den Bossche J  et al. Intracellular trafficking and gene expression of pH-sensitive, artificially enveloped adenoviruses in vitro and in vivo. Biomaterials 32:3085-93 (2011). Dot blot . Read more (PubMed: 21269689) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human infected cell line)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Low pH buffer
    Permeabilization
    No
    Specification
    Human infected cell line
    Blocking step
    Dako Flex peroxidase block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (AD293 CELL)
    Specification
    AD293 CELL
    Fixative
    Methanol
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 08 2013

    I received confirmation from the source of ab24240 that the antibody tested was used with an Alexa Red conjugated secondary antibody. The antibody is unavailable as a conjugated form I'm afraid. I have clarified this information on the legend of image ...

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    Thank you for your email. We do not have exact expiration dates for our products; however, ab8251 should be fine stored at 4C for at least a year. If you have any additional questions, please contact us again.

    Thank you for your enquiry. Unfortunately I do not have details of the degree of reactivity of ab8249 and ab8251 against the Adenovirus subclasses. However, I can tell you that ab7428 recognises all Adenovirus serotypes. All antibodies are yet to...

    Read More

    Unfortunately we have not tested the antibodies in that application. The clone 1E11 is working in ELISA and the clones 7C11 and 8C4 in ELISA and immunodiffusion. This is all the information we currently have on this product.

    This antibody recognises an epitope common to all adenoviruses, therefore it will detect adenovirus 5.

    ab7428, Mouse monoclonal to Adenovirus, recognises a common epitope expressed on all adenoviruses tested and therefore should recognise any virus-infected tissue regardless of species origin.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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