Anti-Adenovirus Type 5 E1A antibody [0.N.8] (ab31686)

Overview

  • Product nameAnti-Adenovirus Type 5 E1A antibody [0.N.8]
    See all Adenovirus Type 5 E1A primary antibodies
  • Description
    Mouse monoclonal [0.N.8] to Adenovirus Type 5 E1A
  • Specificityab31686 recognises Adenovirus, Type 5.
  • Tested applicationsSuitable for: IHC-P, IHC-FoFr, ICC/IF, IP, WB, IHC-Frmore details
  • Species reactivity
    Adenovirus.
  • Immunogen

    Tissue/ cell preparation Adenovirus.

  • Positive control
    • 293 cells or adenovirus infected cells and tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab31686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 2 hours at room temperature.
IHC-FoFr Use a concentration of 2 - 4 µg/ml. Perform antigen retrieval before commencing the IHC protocol. Incubate for 2 hours at room temperature.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 35-46 kDa. Incubate for 2 hours at room temperature.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceThe early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
  • Alternative names
    • Hadv 5 E1A antibody
    • HAdv5 E1A antibody

References for Anti-Adenovirus Type 5 E1A antibody [0.N.8] (ab31686)

This product has been referenced in:
  • Turowski G  et al. Viral infection in placenta relevant cells - a morphological and immunohistochemical cell culture study. APMIS N/A:N/A (2014). Read more (PubMed: 25244625) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Syrian Hamster Tissue sections (Brain. Infected NSC-GFP with adenovirus were injec)
Specification Brain. Infected NSC-GFP with adenovirus were injec
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Bart Thaci

Verified customer

Submitted May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"