Overview

  • Product name
    Anti-Adenovirus Type 5 E1A antibody [M58]
    See all Adenovirus Type 5 E1A primary antibodies
  • Description
    Mouse monoclonal [M58] to Adenovirus Type 5 E1A
  • Specificity
    ab31686 recognises Adenovirus, Type 5. The clone number has been updated from (0.N.8) to (M58) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: IHC-P, IHC-FoFr, ICC/IF, IP, WB, IHC-Frmore details
  • Species reactivity
    Adenovirus.
  • Immunogen

    Tissue/ cell preparation Adenovirus.

  • Positive control
    • 293 cells or adenovirus infected cells and tissues.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab31686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 2 hours at room temperature.
IHC-FoFr Use a concentration of 2 - 4 µg/ml. Perform antigen retrieval before commencing the IHC protocol. Incubate for 2 hours at room temperature.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 35-46 kDa. Incubate for 2 hours at room temperature.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
  • Alternative names
    • Hadv 5 E1A antibody
    • HAdv5 E1A antibody

References for Anti-Adenovirus Type 5 E1A antibody [M58] (ab31686)

This product has been referenced in:
  • Saha B & Parks RJ Human adenovirus type 5 vectors deleted of early region 1 (E1) undergo limited expression of early replicative E2 proteins and DNA replication in non-permissive cells. PLoS One 12:e0181012 (2017). WB ; Human . Read more (PubMed: 28700677) »
  • Turowski G  et al. Viral infection in placenta relevant cells - a morphological and immunohistochemical cell culture study. APMIS N/A:N/A (2014). Read more (PubMed: 25244625) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293)
Permeabilization
Yes - 0.1% Triton X100
Specification
HEK 293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Syrian Hamster Tissue sections (Brain. Infected NSC-GFP with adenovirus were injec)
Specification
Brain. Infected NSC-GFP with adenovirus were injec
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Bart Thaci

Verified customer

Submitted May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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