Anti-Adenovirus Type 5 E1A antibody [M58] (ab76552)

Overview

  • Product nameAnti-Adenovirus Type 5 E1A antibody [M58]
    See all Adenovirus Type 5 E1A primary antibodies
  • Description
    Mouse monoclonal [M58] to Adenovirus Type 5 E1A
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Adenovirus
  • Immunogen

    Adenovirus.

  • Positive control
    • 293 cells or adenovirus-infected cells and tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab76552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
ICC/IF
  • Application notesICC/IF: Use at a concentration of 0.2 - 0.4 µg/ml.
    IHC-P: Use at a concentration of 2 - 4 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min
    followed by cooling at room temperature for 20 min.
    IP: Use at 2µg/mg of lysate.
    WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
    • Alternative names
      • Hadv 5 E1A antibody
      • HAdv5 E1A antibody

    Anti-Adenovirus Type 5 E1A antibody [M58] images

    • Anti-Adenovirus Type 5 E1A antibody [M58] (ab76552) at 0.5 µg/ml + Human cells

      Predicted band size : 32 kDa

    References for Anti-Adenovirus Type 5 E1A antibody [M58] (ab76552)

    ab76552 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"