The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 0.2 - 0.4 µg/ml.
IHC-P: Use at a concentration of 2 - 4 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min
followed by cooling at room temperature for 20 min.
IP: Use at 2µg/mg of lysate.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
Hadv 5 E1A antibody
HAdv5 E1A antibody
Anti-Adenovirus Type 5 E1A antibody [M58] images
Western blot - Adenovirus Type 5 E1A antibody [M58] - BSA and Azide free (ab76552)
Anti-Adenovirus Type 5 E1A antibody [M58] (ab76552) at 0.5 µg/ml + Human cells
Predicted band size : 32 kDa
References for Anti-Adenovirus Type 5 E1A antibody [M58] (ab76552)
has not yet been referenced specifically in any publications.
Publishing research using ab76552? Please let us know so that we can cite the reference in this datasheet.
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