Overview

  • Product nameAnti-Adenylate cyclase 1 antibody
    See all Adenylate cyclase 1 primary antibodies
  • Description
    Rabbit polyclonal to Adenylate cyclase 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Immunogen corresponds to conserved sequence at or near C terminal end of the protein that are unique to rat/mouse Adenylate cyclase 1 protein.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab38331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 127-130 kDa (predicted molecular weight: 124 kDa).Can be blocked with Adenylate cyclase 1 peptide (ab191587).

Target

  • FunctionThis is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.
  • Tissue specificityBrain, retina and adrenal medulla.
  • Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 2 guanylate cyclase domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adcy1 antibody
    • ADCY1_HUMAN antibody
    • adenyl cyclase antibody
    • Adenylate cyclase 1 antibody
    • Adenylate cyclase type 1 antibody
    • Adenylate cyclase type I antibody
    • Adenylyl cyclase 1 antibody
    • ATP pyrophosphate lyase antibody
    • ATP pyrophosphate-lyase 1 antibody
    • Brain adenylate cyclase 1 antibody
    • Ca(2+)/calmodulin-activated adenylyl cyclase antibody
    see all

Anti-Adenylate cyclase 1 antibody images

  • All lanes : Anti-Adenylate cyclase 1 antibody (ab38331) at 1/500 dilution

    Lane 1 : Mouse liver whole cell lysate
    Lane 2 : Mouse hepatocytes whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 124 kDa
    Observed band size : 127 kDa (why is the actual band size different from the predicted?)


    Exposure time : 25 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Adenylate cyclase 1 antibody (ab38331)

This product has been referenced in:
  • Ikoma-Seki K  et al. Role of LRP1 and ERK and cAMP Signaling Pathways in Lactoferrin-Induced Lipolysis in Mature Rat Adipocytes. PLoS One 10:e0141378 (2015). WB ; Rat . Read more (PubMed: 26506094) »
  • Chen C  et al. Estradiol modulates translocator protein (TSPO) and steroid acute regulatory protein (StAR) via protein kinase A (PKA) signaling in hypothalamic astrocytes. Endocrinology 155:2976-85 (2014). WB ; Rat . Read more (PubMed: 24877623) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification liver, hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jan 05 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (membrane fraction of brain tissue)
Loading amount 30 µg
Specification membrane fraction of brain tissue
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Nov 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampus)
Loading amount 5 µg
Specification Hippocampus
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"