The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/500.
FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
Involvement in diseaseDefects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631].
Sequence similaritiesBelongs to the adenylate kinase family.
ab110164, at a 1/50 dilution, staining Adenylate Kinase 1 in paraffin embedded Human lung carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of the immunizing peptide.