The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml. IF/ICC: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
Involvement in diseaseDefects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631].
Sequence similaritiesBelongs to the adenylate kinase family.
ab54824 at 10 ug/ml staining Adenylate Kinase 1 in human Hela cells by Immunocytochemistry / Immunofluorescence.
Western blot - Anti-Adenylate Kinase 1 antibody (ab54824)
Anti-Adenylate Kinase 1 antibody (ab54824) at 1 µg/ml + Adenylate Kinase 1 protein (Human) (ab88049) at 0.01 µg
Secondary Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
References for Anti-Adenylate Kinase 1 antibody (ab54824)
This product has been referenced in:
Lewis C et al. Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins. J Biomed Biotechnol2010:648501 (2010).
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