Overview

  • Product nameAnti-Adenylate Kinase 1 antibody
    See all Adenylate Kinase 1 primary antibodies
  • Description
    Mouse monoclonal to Adenylate Kinase 1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-195 of Human Adenylate Kinase 1

Properties

Applications

Our Abpromise guarantee covers the use of ab54824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IF/ICC: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
    • Involvement in diseaseDefects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631].
    • Sequence similaritiesBelongs to the adenylate kinase family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adenylate kinase 1 antibody
      • Adenylate kinase isoenzyme 1 antibody
      • Adenylate kinase soluble antibody
      • AK 1 antibody
      • Ak1 antibody
      • ATP AMP transphosphorylase antibody
      • ATP-AMP transphosphorylase 1 antibody
      • KAD1 antibody
      • KAD1_HUMAN antibody
      • Myokinase antibody
      see all

    Anti-Adenylate Kinase 1 antibody images



    • Predicted band size : 22 kDa
      Adenylate Kinase 1 antibody (ab54824) at 1ug/lane + HeLa cell lysate at 25ug/lane.
    • ab54824 at 10 ug/ml staining Adenylate Kinase 1 in human Hela cells by Immunocytochemistry / Immunofluorescence.

    • Anti-Adenylate Kinase 1 antibody (ab54824) at 1 µg/ml + Adenylate Kinase 1 protein (Human) (ab88049) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 2 minutes

    References for Anti-Adenylate Kinase 1 antibody (ab54824)

    This product has been referenced in:
    • Lewis C  et al. Proteomic Profiling of the Dystrophin-Deficient MDX Heart Reveals Drastically Altered Levels of Key Metabolic and Contractile Proteins. J Biomed Biotechnol 2010:648501 (2010). WB ; Mouse . Read more (PubMed: 20508850) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab54824.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"