Anti Perilipin-2 antibody [2C5A3] (ab181463)
Key features and details
- Mouse monoclonal [2C5A3] to Perilipin-2
- Suitable for: IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti Perilipin-2 antibody [2C5A3]
See all Perilipin-2 primary antibodies -
Description
Mouse monoclonal [2C5A3] to Perilipin-2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Perilipin-2 aa 250 to the C-terminus. (Expressed in E.coli).
Database link: Q99541 -
Positive control
- Perilipin-2 recombinant protein; Perilipin-2 (Amino acids: 286-437)-hIgGFc transfected HEK293 cell lysate; Human liver cancer and esophageal cancer tissues.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR154667-17 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2C5A3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 48 kDa.
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Notes |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 48 kDa. |
Target
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Function
May be involved in development and maintenance of adipose tissue. -
Tissue specificity
Milk lipid globules. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsAcylated; primarily with C14, C16 and C18 fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 123 Human
- Omim: 103195 Human
- SwissProt: Q99541 Human
- Unigene: 3416 Human
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Alternative names
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling Perilipin-2 with ab181463 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Perilipin-2 with ab181463 at 1/200 dilution.
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All lanes : Anti Perilipin-2 antibody [2C5A3] (ab181463) at 1/500 dilution
Lane 1 : HEK293 cell lysate.
Lane 2 : Perilipin-2 (Amino acids : 286-437)-hIgGFc transfected HEK293 cell lysate.
Predicted band size: 48 kDa -
Anti Perilipin-2 antibody [2C5A3] (ab181463) at 1/500 dilution + Perilipin-2 recombinant protein.
Predicted band size: 48 kDaExpected MWt is 42.6 kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab181463 has been referenced in 2 publications.
- Zhang X et al. Abnormal lipid metabolism in epidermal Langerhans cells mediates psoriasis-like dermatitis. JCI Insight 7:N/A (2022). PubMed: 35801590
- Schipke J et al. Lipofibroblasts in Structurally Normal, Fibrotic, and Emphysematous Human Lungs. Am J Respir Crit Care Med 204:227-230 (2021). PubMed: 34015242