Anti Perilipin-2 antibody [2C5H8] (ab181452)
Key features and details
- Mouse monoclonal [2C5H8] to Perilipin-2
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti Perilipin-2 antibody [2C5H8]
See all Perilipin-2 primary antibodies -
Description
Mouse monoclonal [2C5H8] to Perilipin-2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human Perilipin-2 aa 250 to the C-terminus. Expressed in E. coli.
Database link: Q99541 -
Positive control
- Perilipin-2 recombinant protein; Perilipin-2 transfected HEK293 cell lysate; HepG2 cells and cell lysate; Human rectum cancer tissue; Human bladder cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR197175-12 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% stabilization buffer (amino acid =85% pH(1% water solution) =7.0~7.5, Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2C5H8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000.
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ICC/IF |
1/200 - 1/1000.
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|
Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. |
ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
May be involved in development and maintenance of adipose tissue. -
Tissue specificity
Milk lipid globules. -
Sequence similarities
Belongs to the perilipin family. -
Post-translational
modificationsAcylated; primarily with C14, C16 and C18 fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 280981 Cow
- Entrez Gene: 123 Human
- Omim: 103195 Human
- SwissProt: Q9TUM6 Cow
- SwissProt: Q99541 Human
- SwissProt: Q4PLW0 Pig
- Unigene: 3416 Human
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Alternative names
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
Images
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Anti Perilipin-2 antibody [2C5H8] (ab181452) at 1/500 dilution + HepG2 cell lysate
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All lanes : Anti Perilipin-2 antibody [2C5H8] (ab181452) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Perilipin-2-transfected HEK293 cell lysate -
Anti Perilipin-2 antibody [2C5H8] (ab181452) at 1/500 dilution + Perilipin-2 recombinant protein
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Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Perilipin-2 with ab181452 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Perilipin-2 with ab181452 at 1/200 dilution.
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Immunofluorescent analysis of HepG2 cells labeling Perilipin-2 with ab181452 (green) at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye.
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Flow cytometric analysis of HepG2 cells labeling Perilipin-2 with ab181452 (green) at 1/200 dilution, and negative control (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab181452 has been referenced in 4 publications.
- He Y et al. Lipid Droplet-Related PLIN2 in CD68+ Tumor-Associated Macrophage of Oral Squamous Cell Carcinoma: Implications for Cancer Prognosis and Immunotherapy. Front Oncol 12:824235 (2022). PubMed: 35372038
- Su W et al. Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice. Nat Commun 13:6577 (2022). PubMed: 36323699
- Yuan Y et al. Mitochondrial E3 ubiquitin ligase 1 promotes autophagy flux to suppress the development of clear cell renal cell carcinomas. Cancer Sci 110:3533-3542 (2019). PubMed: 31489722
- Carrillo M et al. Optical tissue clearing in combination with perfusion and immunofluorescence for placental vascular imaging. Medicine (Baltimore) 97:e12392 (2018). PubMed: 30278515