Anti-ADFP antibody [2C5H8] (ab181452)

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Product name

Anti-ADFP antibody [2C5H8]
See all ADFP primary antibodies
Description

Mouse monoclonal [2C5H8] to ADFP
Host species

Mouse
Tested applications

Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
Species reactivity

Reacts with: Human
Predicted to work with: Cow, Pig
Immunogen

Recombinant fragment corresponding to Human ADFP aa 268-437. Expressed in E. coli.
Sequence:
VEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGV PQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESL DDVMDYLVNNTPLNWLVGPFYPQLT ESQNAQDQGA EMDKSSQETQ RSEHKTH

Database link: Q99541

Run BLAST with Run BLAST with
Positive control
- ADFP recombinant protein; ADFP transfected HEK293 cell lysate; HepG2 cells and cell lysate; Human rectum cancer tissue; Human bladder cancer tissue.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR197175-12 are from Tissue Culture Supernatant

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS

0.5% stabilization buffer (amino acid =85% pH(1% water solution) =7.0~7.5, Water =2% As(mg/kg) =0.5 Pb(mg/kg) =0.1)
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

2C5H8
Isotype

IgG1
Research areas

Our Abpromise guarantee covers the use of ab181452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/200 - 1/1000.
WB		1/500 - 1/2000.
ICC/IF		1/200 - 1/1000.
Flow Cyt		1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Function

May be involved in development and maintenance of adipose tissue.
Tissue specificity

Milk lipid globules.
Sequence similarities

Belongs to the perilipin family.
Post-translational
modifications

Acylated; primarily with C14, C16 and C18 fatty acids.
Cellular localization

Membrane.
Target information above from: UniProt accession Q99541 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 280981 Cow
- Entrez Gene: 123 Human
- Omim: 103195 Human
- SwissProt: Q9TUM6 Cow
- SwissProt: Q99541 Human
- SwissProt: Q4PLW0 Pig
- Unigene: 3416 Human
Alternative names
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
- Adipose differentiation-related protein antibody
- ADRP antibody
- MGC10598 antibody
- Perilipin-2 antibody
- PLIN2 antibody
- PLIN2_HUMAN antibody
see all

Western blot - Anti-ADFP [2C5H8] antibody (ab181452)

Anti-ADFP antibody [2C5H8] (ab181452) at 1/500 dilution + HepG2 cell lysate
Western blot - Anti-ADFP [2C5H8] antibody (ab181452)

All lanes : Anti-ADFP antibody [2C5H8] (ab181452) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : ADFP-transfected HEK293 cell lysate
Western blot - Anti-ADFP [2C5H8] antibody (ab181452)

Anti-ADFP antibody [2C5H8] (ab181452) at 1/500 dilution + ADFP recombinant protein
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP [2C5H8] antibody (ab181452)

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling ADFP with ab181452 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADFP [2C5H8] antibody (ab181452)

Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling ADFP with ab181452 at 1/200 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ADFP [2C5H8] antibody (ab181452)

Immunofluorescent analysis of HepG2 cells labeling ADFP with ab181452 (green) at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry - Anti-ADFP [2C5H8] antibody (ab181452)

Flow cytometric analysis of HepG2 cells labeling ADFP with ab181452 (green) at 1/200 dilution, and negative control (red).

ab181452 has not yet been referenced specifically in any publications.

Publishing research using ab181452? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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