Overview

  • Product nameAnti-ADFP antibody
    See all ADFP primary antibodies
  • Description
    Rabbit polyclonal to ADFP
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Monkey
  • Immunogen

    A synthetic peptide made to an internal portion of the human ADFP protein, within residues 150-250.

  • Positive control
    • Human liver lysate

Applications

Our Abpromise guarantee covers the use of ab52355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19920176
WB Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

Anti-ADFP antibody images

  • Anti-ADFP antibody (ab52355) at 2 µg/ml + Human liver lysate

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab52355 staining ADFP in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50: in PBS; 1% BSA) for 1 hour at 25°C. An Alexa Fluor®568-conjugated Goat polyclonal to rabbit IgG, dilution 1/100, was used as secondary antibody.

    See Abreview

References for Anti-ADFP antibody (ab52355)

This product has been referenced in:
  • Akil A  et al. Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV. Nat Commun 7:12203 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27417143) »
  • Eggert D  et al. Visualization and analysis of hepatitis C virus structural proteins at lipid droplets by super-resolution microscopy. PLoS One 9:e102511 (2014). ICC/IF . Read more (PubMed: 25019511) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 1 µg
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Human Cell lysate - whole cell (Huh7 liver)
Specification Huh7 liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 14 2014

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% saponin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Francesca Lolicato

Verified customer

Submitted Jan 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"