Overview

  • Product nameAnti-ADFP antibody
    See all ADFP primary antibodies
  • Description
    Rabbit polyclonal to ADFP
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ADFP.

    (Peptide available as ab90212.)

  • Positive control
    • Ab78920 gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293. This antibody also gave a positive signal in Mouse Heart Tissue Lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab78920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

Anti-ADFP antibody images

  • All lanes : Anti-ADFP antibody (ab78920) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab78920 stained HepG2 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78920, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa, and Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, and HepG2 cells at 1µg/ml
  • Anti-ADFP antibody (ab78920) at 1 µg/ml + Heart (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa


    Exposure time : 2 minutes
  • Anti-ADFP antibody (ab78920) at 1 µg/ml + Recombinant Human ADFP protein (ab73631) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-ADFP antibody (ab78920)

ab78920 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Sample African Green Monkey Cell (Rhesus monkey kidney cells)
Specification Rhesus monkey kidney cells
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Huh7)
Loading amount 50 µg
Specification Huh7
Treatment siADRP 10uM
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Alina Macovei

Verified customer

Submitted Feb 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepaRG)
Specification HepaRG
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Alina Macovei

Verified customer

Submitted Jun 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"