Overview

  • Product nameAnti-ADH1B antibody [4F12]
    See all ADH1B primary antibodies
  • Description
    Mouse monoclonal [4F12] to ADH1B
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human
  • Immunogen

    Recombinant full length Human ADH1B (NP_000659) produced in HEK293T cells.

  • Positive control
    • WB: HEK293T cell lysate transfected with pCMV6-ENTRY ADH1B cDNA; HeLa, SVT2, Jurkat, MDCK, PC12 and MCF7 cell extracts; IHC-P: Human breast adenocarcinoma, colon adenocarcinoma, liver, lung, ovary adenocarcinoma thyroid carcinoma, lymph node and lymphoma tissues; ICC/IF:COS7 cells transiently transfected by pCMV6-ENTRY ADH1B, HeLa cells; Flow Cyt: HEK293T cells transfected with an ADH1B overexpress plasmid, Jurkat cells
  • General notesDilute in PBS (pH 7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab123905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 40 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH beta subunit antibody
    • ADH1B antibody
    • ADH1B_HUMAN antibody
    • ADH2 antibody
    • Alcohol dehydrogenase 1B antibody
    • alcohol dehydrogenase 2 (class I), beta polypeptide antibody
    • Alcohol dehydrogenase 2 antibody
    • Alcohol dehydrogenase subunit beta antibody
    • Aldehyde reductase antibody
    • DKFZp686C06125 antibody
    • OTTHUMP00000220192 antibody
    see all

Anti-ADH1B antibody [4F12] images

  • All lanes : Anti-ADH1B antibody [4F12] (ab123905) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ADH1B cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205391)
  • All lanes : Anti-ADH1B antibody [4F12] (ab123905) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 40 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205391)
  • ab123905, at a 1/150 dilution, staining ADH1B in paraffin embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
  • ab123905, at a 1/150 dilution, staining ADH1B in paraffin embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab123905, at a 1/150 dilution, staining ADH1B in paraffin embedded Human liver tissue by Immunohistochemistry.
  • ab123905, at a 1/150 dilution, staining ADH1B in paraffin embedded Human lung tissue by Immunohistochemistry.
  • ab123905, at a 1/150 dilution, staining ADH1B in paraffin embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab123905, at a 1/150 dilution, staining ADH1B in paraffin embedded Human thyroid carcinoma tissue by Immunohistochemistry.
  • ab123905, at a 1/150 dilution, staining ADH1B in paraffin embedded Human lymph node tissue by Immunohistochemistry.
  • ab123905, at a 1/150 dilution, staining ADH1B in paraffin embedded Human lymphoma tissue by Immunohistochemistry.
  • ab123905, at a 1/100 dilution, staining ADH1B in COS7 cells transiently transfected with pCMV6-ENTRY ADH1B by Immunofluorescence.
  • ab123905, at 1/100 dilution, staining ADH1B in HeLa cells by Immunofluorescence.
  • ab123905, at a 1/100 dilution, staining ADH1B in HEK293T cells transfected with an ADH1B overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry.
  • ab123905, at a 1/100 dilution, staining ADH1B in HeLa cells (Red) by Flow Cytometry, compared to a nonspecific negative control antibody (Blue).
  • ab123905, at a 1/100 dilution, staining ADH1B in Jurkat cells (Red) by Flow Cytometry, compared to a nonspecific negative control antibody (Blue).

References for Anti-ADH1B antibody [4F12] (ab123905)

ab123905 has not yet been referenced specifically in any publications.

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