Overview

  • Product name
  • Description
    Rabbit polyclonal to ADH1B
  • Specificity
    This antibody reacts with ADH1B
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    A region within synthetic peptide: TSVIVGVPPA SQNLSINPML LLTGRTWKGA VYGGFKSKEG IPKLVADFMA, corresponding to C terminal amino acids 289-338 of Human ADH1B

  • Positive control
    • Jurkat cell lysate and human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab58647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Sequence similarities
    Belongs to the zinc-containing alcohol dehydrogenase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH beta subunit antibody
    • ADH1B antibody
    • ADH1B_HUMAN antibody
    • ADH2 antibody
    • Alcohol dehydrogenase 1B antibody
    • alcohol dehydrogenase 2 (class I), beta polypeptide antibody
    • Alcohol dehydrogenase 2 antibody
    • Alcohol dehydrogenase subunit beta antibody
    • Aldehyde reductase antibody
    • DKFZp686C06125 antibody
    • OTTHUMP00000220192 antibody
    see all

Images

  • Anti-ADH1B antibody (ab58647) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ADH1B with ab58647 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.

References

ab58647 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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