Overview

  • Product name
  • Description
    Rabbit polyclonal to ADH1B
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Chimpanzee, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (STAGKVIKCK AAVLWEVKKP FSIEDVEVAP PKAYEVRIKM VAVGICHTDD) of Human ADH1B (NP_000659).

  • Positive control
    • Human fetal lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • Sequence similarities
    Belongs to the zinc-containing alcohol dehydrogenase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH beta subunit antibody
    • ADH1B antibody
    • ADH1B_HUMAN antibody
    • ADH2 antibody
    • Alcohol dehydrogenase 1B antibody
    • alcohol dehydrogenase 2 (class I), beta polypeptide antibody
    • Alcohol dehydrogenase 2 antibody
    • Alcohol dehydrogenase subunit beta antibody
    • Aldehyde reductase antibody
    • DKFZp686C06125 antibody
    • OTTHUMP00000220192 antibody
    see all

Images

  • Anti-ADH1B antibody (ab83475) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

References

ab83475 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83475.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up