Recombinant
RabMAb

Anti-ADH5 antibody [EPR12885(B)] (ab177932)

Overview

  • Product name
    Anti-ADH5 antibody [EPR12885(B)]
    See all ADH5 primary antibodies
  • Description
    Rabbit monoclonal [EPR12885(B)] to ADH5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ADH5 aa 1-100. The exact sequence is proprietary.
    Database link: P11766

  • Positive control
    • Human fetal liver, HepG2, Human fetal kidney and SH-SY5Y lysates. Human brain and kidney tissue. Permeabilized SH-SY5Y cells. Immunoprecipitation pellet from Human fetal liver lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
    • Sequence similarities
      Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADH 3 antibody
      • ADH5 antibody
      • ADHX antibody
      • ADHX_HUMAN antibody
      • Alcohol dehydrogenase (class III) chi polypeptide antibody
      • alcohol dehydrogenase 5 (class III) chi polypeptide antibody
      • Alcohol dehydrogenase 5 antibody
      • Alcohol dehydrogenase class 3 antibody
      • Alcohol dehydrogenase class chi chain antibody
      • Alcohol dehydrogenase class III antibody
      • Alcohol dehydrogenase class-3 antibody
      • Alcohol dehydrogenase class-III antibody
      • class III alcohol dehydrogenase 5 chi subunit antibody
      • FALDH antibody
      • FDH antibody
      • formaldehyde dehydrogenase antibody
      • Glutathione dependent formaldehyde dehydrogenase antibody
      • Glutathione-dependent formaldehyde dehydrogenase antibody
      • GSH-FDH antibody
      • GSNOR antibody
      • hydroxymethyllutathione dehydrogenase antibody
      • S-(hydroxymethyl)glutathione dehydrogenase antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with ab177932 at a 1/50 dilution.

    • All lanes : Anti-ADH5 antibody [EPR12885(B)] (ab177932) at 1/1000 dilution

      Lane 1 : Human fetal liver lysates
      Lane 2 : HepG2 lysates
      Lane 3 : Human fetal kidney lysates
      Lane 4 : SH-SY5Y lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 40 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with ab177932 at a 1/50 dilution.

    • Flow cytometric analysis of permeabilized SH-SY5Y cells using ab177932 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver lysate or (Lane 2) 1XPBS (negative control) using ab177932 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab177932 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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