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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ADH5 aa 1-100. The exact sequence is proprietary.
Database link: P11766
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab177932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ADH5 with ab177932 at a 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADH5 with ab177932 at a 1/50 dilution.
Flow cytometric analysis of permeabilized SH-SY5Y cells using ab177932 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver lysate or (Lane 2) 1XPBS (negative control) using ab177932 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab177932 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"