Overview

  • Product nameAdiponectin Human ELISA KitSee all Adiponectin kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Recovery

    < 96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.59 83% - 107%
    Serum 93.57 83% - 104%
    Plasma 90.29 80% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Adiponectin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Adiponectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Adiponectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Adiponectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adiponectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificitySynthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in diseaseAdiponectin deficiency (ADPND) [MIM:612556]: A condition that results in very low concentrations of plasma adiponectin. Note=The disease is caused by mutations affecting the gene represented in this entry.
    Diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • Sequence similaritiesContains 1 C1q domain.
    Contains 1 collagen-like domain.
  • DomainThe C1q domain is commonly called the globular domain.
  • Post-translational
    modifications
    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • 30 kDa adipocyte complement related protein
    • 30 kDa adipocyte complement-related protein
    • ACDC
    • ACRP30
    • ADIPO_HUMAN
    • Adipocyte
    • Adipocyte C1q and collagen domain containing protein
    • Adipocyte complement related 30 kDa protein
    • Adipocyte complement related protein of 30 kDa
    • Adipocyte complement-related 30 kDa protein
    • Adiponectin
    • Adipoq
    • Adipose most abundant gene transcript 1
    • Adipose most abundant gene transcript 1 protein
    • Adipose specific collagen like factor
    • ADIPQTL1
    • ADPN
    • apM-1
    • APM1
    • C1q and collagen domain-containing protein
    • GBP28
    • Gelatin binding protein
    • Gelatin binding protein 28
    • Gelatin-binding protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Adiponectin Human ELISA Kit images

  • Human Adiponectin measured in biological fluids showing quantity (pg) per mL of tested sample.
  • Representative Standard Curve using ab99968

  • Representative Standard Curve using ab99968

Protocols

References for Adiponectin Human ELISA Kit (ab99968)

This product has been referenced in:
  • Schlottmann I  et al. Calcium-dependent release of adipocyte fatty acid binding protein from human adipocytes. Int J Obes (Lond) N/A:N/A (2013). ELISA ; Human . Read more (PubMed: 24352293) »
  • Bilwani FA & Knight KL Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis. J Immunol 189:4379-86 (2012). ELISA ; Human . Read more (PubMed: 23002443) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. I sent your inquiry to the lab, they did not send original data but sent the reply below. Please let me know if you have any more questions.



. A 30,000-fold dilution is quite high but our Human IgG ELISA ...

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Thank you for contacting Abcam.

Unfortunately we have not tested pig samples with theab99968 Human Adiponectin ELISA kit, so we do not know the cross reactivity.Both antibodies used in this kit are mouse monoclonal and the epitope recognized ...

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Thank you for your enquiry.

It is possible that high viscosity of the sample can cause interference with the results. However, I would like to state that we have not tested media with such a high fat content and viscosity, therefore we do no...

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I'm happy to help! Please let me know if you need anything further.

The biotinylated anti-human adiponectin antibody is monoclonal and is raised from mice.

I hope this is helpful. Please contact us again if you have any further questions.

Thank you for your reply and for your patience. It has taken a day or two to confirm this information for you.

ab99968 Adiponectin Human ELISA Kitas been successfully tested with EDTA-plasma, heparin-plasma, and citrate-plasma.

Ot...

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Thank you for your recent telephone enquiry.

I can confirm that the matched pair of antibodies for the Human Adiponectin ELISA kit (ab99968) have an epitope in the globular region (aa 104-244). Therefore, this kit will recognize trimers and ...

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Thank you for contacting us. The capture antibody for the Adiponectin Human ELISA Kits are a monoclonal mouse antibody for kit ab99968 and a polyclonal antibody for kit ab108786 I hope this information is helpful to you. Please do not h...

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