Adiponectin Human ELISA Kit (ab99968)
- Product nameAdiponectin Human ELISA KitSee all Adiponectin kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity< 25 pg/ml
< 96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.59 83% - 107% Serum 93.57 83% - 104% Plasma 90.29 80% - 103%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Human Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Adiponectin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Adiponectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Adiponectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Adiponectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adiponectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 80x HRP-Streptavidin Concentrate 1 x 200µl Adiponectin Microplate with anti-human Adiponectin. 1 unit Assay Diluent A 2 x 30ml Assay Diluent C 2 x 15ml biotinylated antihuman Adiponectin 2 vials Standards recombinant human Adiponectin. 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
- FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
- Tissue specificitySynthesized exclusively by adipocytes and secreted into plasma.
- Involvement in diseaseAdiponectin deficiency (ADPND) [MIM:612556]: A condition that results in very low concentrations of plasma adiponectin. Note=The disease is caused by mutations affecting the gene represented in this entry.
Diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
- Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain.
- DomainThe C1q domain is commonly called the globular domain.
modificationsHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
- Cellular localizationSecreted.
- 30 kDa adipocyte complement related protein
- 30 kDa adipocyte complement-related protein
- Adipocyte C1q and collagen domain containing protein
- Adipocyte complement related 30 kDa protein
- Adipocyte complement related protein of 30 kDa
- Adipocyte complement-related 30 kDa protein
- Adipose most abundant gene transcript 1
- Adipose most abundant gene transcript 1 protein
- Adipose specific collagen like factor
- C1q and collagen domain-containing protein
- Gelatin binding protein
- Gelatin binding protein 28
- Gelatin-binding protein
Our Abpromise guarantee covers the use of ab99968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Adiponectin Human ELISA Kit images
Representative Standard Curve using ab99968
Representative Standard Curve using ab99968
References for Adiponectin Human ELISA Kit (ab99968)
This product has been referenced in:
- Bilwani FA & Knight KL Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis. J Immunol 189:4379-86 (2012). ELISA ; Human . Read more (PubMed: 23002443) »