• Product nameAdiponectin Rat ELISA kit
    See all Adiponectin kits
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.56 ng/ml - 100 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s Adiponectin Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of rat Adiponectin in urine, plasma, serum, and cell culture supernatants.

    An Adiponectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Adiponectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adiponectin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate


  • FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificitySynthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in diseaseDefects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similaritiesContains 1 C1q domain.
    Contains 1 collagen-like domain.
  • DomainThe C1q domain is commonly called the globular domain.
  • Post-translational
    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • 30 kDa adipocyte complement related protein
    • 30 kDa adipocyte complement-related protein
    • ACDC
    • ACRP30
    • Adipocyte
    • Adipocyte C1q and collagen domain containing protein
    • Adipocyte complement related 30 kDa protein
    • Adipocyte complement related protein of 30 kDa
    • Adipocyte complement-related 30 kDa protein
    • adipocyte-specific secretory protein
    • Adiponectin
    • Adiponectin precursor
    • adiponectin, C1Q and collagen domain containing
    • Adipoq
    • Adipose most abundant gene transcript 1
    • Adipose most abundant gene transcript 1 protein
    • Adipose specific collagen like factor
    • ADIPQTL1
    • ADPN
    • APM 1
    • apM-1
    • APM1
    • C1q and collagen domain-containing protein
    • GBP28
    • Gelatin binding protein
    • Gelatin binding protein 28
    • Gelatin-binding protein
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Adiponectin Rat ELISA kit images

  • Adiponectin measured in tissue lysates showing quantity (ng) per 1 million cells

  • Representative Standard Curve using ab108784.


References for Adiponectin Rat ELISA kit (ab108784)

This product has been referenced in:
  • Youcef G  et al. Simultaneous characterization of metabolic, cardiac, vascular and renal phenotypes of lean and obese SHHF rats. PLoS One 9:e96452 (2014). Read more (PubMed: 24831821) »
  • Hajje G  et al. Hypothyroidism and its rapid correction alter cardiac remodeling. PLoS One 9:e109753 (2014). Sandwich ELISA ; Rat . Read more (PubMed: 25333636) »

See all 2 Publications for this product

Product Wall

Standard in this kit was 1.6 ug/bottle. The customer should add 4 ml to get standard solution of 400 ng/ml and then do 1:4 dilutions for the first standard point to get working solution of 100 ng/ml

We sold out all kits from this lot. We have...

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Abreview for Adiponectin Rat ELISA kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Rat Serum (Blood)
Specification: Blood
Amount of Sample: 50 µg
Type of Kit Used: ELISA
Positive control: rat serum (diabetic)
Did you use the Abcam Kit protocol?: Yes

Dr. Asmat Salim

Verified customer

Submitted May 07 2012

Thank you for contacting us.

Please dilute all the reagents in diluted diluent e.g. 1X Diluent.

I would recommend diluting the required amount based on the volume used for the assay. The diluted diluent will be stable for 1 month ...

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Thank you for contacting Abcam regarding this issue. It is okay to use heparin coated tubes and avoid having to use the 0.1M sodium citrate. I hope this helps. If there is anything else that I can help you with please let me know.

Thank you for contacting Abcam. According to the datasheet for ab108784, plasma samples can be stored at -20C or below for 3 months. Please let me know if there is anything else I can help you with.