Anti-Adiponectin Receptor 1 antibody (ab77611)

Overview

  • Product nameAnti-Adiponectin Receptor 1 antibody
    See all Adiponectin Receptor 1 primary antibodies
  • Description
    Goat polyclonal to Adiponectin Receptor 1
  • SpecificityRecognizes Human ADPR1 Receptor. Not likely to cross react with ADPR2.
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Cat, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    CYAARIPERFFPGKFDIWFQSHQIF

    , corresponding to C terminal amino acids 317-340 of Human Adiponectin Receptor 1

  • Positive control
    • Human liver tissue and human muscle tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab77611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
  • Application notesICC: 1/400 (acetone fixed cells).
    IHC-P: 1/250. Recommended buffer: 10 mM KHPO4, 140 mM NaCl, pH 7.2.
    WB: 1/1000. Predicted molecular weight: 42 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.
    • Tissue specificityWidely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.
    • Sequence similaritiesBelongs to the ADIPOR family.
    • DomainThe N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
    • Cellular localizationMembrane. Localized to the cell membrane and intracellular organelles.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACDCR1 antibody
      • ADIPO R1 antibody
      • Adiponectin receptor protein 1 antibody
      • ADIPOR 1 antibody
      • Adipor1 antibody
      • ADR1_HUMAN antibody
      • CGI 45 antibody
      • CGI 45 protein antibody
      • CGI-45 antibody
      • CGI45 antibody
      • CGI45 protein antibody
      • FLJ25385 antibody
      • FLJ42464 antibody
      • PAQR1 antibody
      • Progestin and adipoQ receptor family member I antibody
      • TESBP1A antibody
      see all

    Anti-Adiponectin Receptor 1 antibody images

    • Paraffin section of human liver tissue stained using antibody to human ADPR1 receptor C-terminus ab77611 at 1/250 dilution.
    • Paraffin section of human muscle tissue stained using antibody to human ADPR1 receptor C-terminus ab77611 at 1/250 dilution.

    References for Anti-Adiponectin Receptor 1 antibody (ab77611)

    ab77611 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Liver)
    Specification Liver
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step Normal goat serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 08 2010

    Application Flow Cytometry
    Sample Human Cell (mesothelioma)
    Specification mesothelioma
    Fixation non-fix
    Permeabilization No
    Gating Strategy R2
    Username

    Abcam user community

    Verified customer

    Submitted Jan 27 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"