Recombinant
RabMAb

Anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611)

Overview

  • Product name
    Anti-Adiponectin Receptor 1 antibody [EPR6626]
    See all Adiponectin Receptor 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6626] to Adiponectin Receptor 1
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Adiponectin Receptor 1 aa 1-100 (intracellular).

  • Positive control
    • WB: Rat and mouse heart tissue lysates and human skeletal muscle tissue lysate. IHC-P: Human kidney tissue. ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.

 

IHC-P 1/20 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt 1/30 - 1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50 - 1/400.

 

Target

  • Function
    Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.
  • Tissue specificity
    Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.
  • Sequence similarities
    Belongs to the ADIPOR family.
  • Domain
    The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
  • Cellular localization
    Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACDCR1 antibody
    • ADIPO R1 antibody
    • Adiponectin receptor protein 1 antibody
    • ADIPOR 1 antibody
    • Adipor1 antibody
    • ADR1_HUMAN antibody
    • CGI 45 antibody
    • CGI 45 protein antibody
    • CGI-45 antibody
    • CGI45 antibody
    • CGI45 protein antibody
    • FLJ25385 antibody
    • FLJ42464 antibody
    • PAQR1 antibody
    • Progestin and adipoQ receptor family member I antibody
    • TESBP1A antibody
    see all

Images

  • All lanes : Anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) at 1/1000 dilution (Purified)

    Lane 1 : Mouse heart
    Lane 2 : Rat heart

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 43 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    5% NFDM/TBST dilution buffer

  • Immunohistochemical staining of paraffin embedded human endometrium with purified ab126611 at a dilution of 1/200. The seconday was a HRP polymer for rabbit IgG amd the counter stain used was hematoxylin.

  • Immunocytochemistry of HeLa cells fixed in 4% PFA with purified ab126611 at a dilution of 1/400. An Alexa Fluor® goat anti-rabbit antibody was used as the secondary and the sample was counter stained wtih DAPI.

  • Overlay histogram showing HepG2 cells stained with unpurified ab126611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126611, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemical staining of Adiponectin Receptor 1 in formalin-fixed, paraffin-embedded human kidney tissue by unpurified ab126611, at 1/50 dilution.

  • Anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µg

    Secondary
    HRP anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

    Predicted band size : 43 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Equilibrium disassociation constant (KD) with unpurified ab126611.
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Li X  et al. Expression of Adiponectin Receptor-1 and Prognosis of Epithelial Ovarian Cancer Patients. Med Sci Monit 23:1514-1521 (2017). Read more (PubMed: 28356549) »
  • Fu S  et al. Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity. Sci Rep 7:43771 (2017). Read more (PubMed: 28256562) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you fory our enquiry.

I am pleased to help answer your questions:

1. Based on sequence analysis, there does not appear to be homology between the immunogen sequence for this antibody and ADIPOR2. Therefore, the antibody is n...

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Thank you for contacting us and your interest in our products.

As you have seen on the datasheets for the anti-Adiponectin Receptor 2 antibody (ab77612) and anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) have both been used in flow ...

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Thank you for contacting Abcam regarding our Anti-Adiponectin Receptor 1 antibodies.


Regarding ab126611, this is a rabbit monoclonal antibody and [EPR6626] represents the clone number.


In terms of the differences between...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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