Anti-Adiponectin Receptor 2 antibody (ab77612)


  • Product name
    Anti-Adiponectin Receptor 2 antibody
    See all Adiponectin Receptor 2 primary antibodies
  • Description
    Goat polyclonal to Adiponectin Receptor 2
  • Specificity
    Recognizes Human, Rat, Mouse and Macaca ADPR2 Receptor. May cross react with ADPR1 Receptor.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Macaque monkey
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 362-380 of Human Adiponectin Receptor 2

  • Positive control
    • Human liver and human muscle tissue
  • General notes

    This antibody may be used in developing sandwich ELISA assays in combination with antibodies specific to N terminus of ADPR2 Receptor



Our Abpromise guarantee covers the use of ab77612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/250.

Buffer used was: 10 mM KHPO4, 140 mM NaCl, pH 7.2.

ICC 1/400.

Acetone fixed cells.

Flow Cyt Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • Function
    Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.
  • Tissue specificity
    Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung.
  • Sequence similarities
    Belongs to the ADIPOR family.
  • Domain
    The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
  • Cellular localization
    Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt
  • Database links
  • Alternative names
    • PAQR2 antibody
    • ACDCR2 antibody
    • Adiponectin receptor protein 2 antibody
    • Adipor2 antibody
    • ADR2_HUMAN antibody
    • Progestin and adipoQ receptor family member II antibody
    see all

Anti-Adiponectin Receptor 2 antibody images

  • Paraffin section of human liver tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution.
  • Paraffin section of human muscle tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution.
  • Anti-Adiponectin Receptor 2 antibody (ab77612) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Exposure time : 20 minutes

References for Anti-Adiponectin Receptor 2 antibody (ab77612)

This product has been referenced in:
  • Basu S  et al. Cellular Expression of Cyclooxygenase, Aromatase, Adipokines, Inflammation and Cell Proliferation Markers in Breast Cancer Specimen. PLoS One 10:e0138443 (2015). IHC-Fr ; Human . Read more (PubMed: 26431176) »
  • Wu U  et al. Protective effects of berberine on high fat-induced kidney damage by increasing serum adiponectin and promoting insulin sensitivity. Int J Clin Exp Pathol 8:14486-92 (2015). Read more (PubMed: 26823767) »

See all 4 Publications for this product

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As you have seen on the datasheets for the anti-Adiponectin Receptor 2 antibody (ab77612) and anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) have both been used in flow ...

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Western blot
Pig Cell lysate - whole cell (porcine skeletal muscle)
Loading amount
50 µg
porcine skeletal muscle
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
RotiBlock (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) in TBS (Tris buffered saline, 200 mM Tris, 1.37 M NaCl, pH 7.4) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Blocking step
Normal goat serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Flow Cytometry
Human Cell (mesothelioma cell line (ATCC: MSTO-211H))
mesothelioma cell line (ATCC: MSTO-211H)
Cell harvesting/tissue preparation method: 10% FCS / RPMI 1640
Sample buffer: 2% FCS / PBS
Gating Strategy
R1 and R2

Abcam user community

Verified customer

Submitted Feb 22 2010


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