Anti-Adipose Triglyceride Lipase antibody (ab85858)

Overview

  • Product nameAnti-Adipose Triglyceride Lipase antibody
    See all Adipose Triglyceride Lipase primary antibodies
  • Description
    Goat polyclonal to Adipose Triglyceride Lipase
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Pig
  • Immunogen

    Synthetic peptide:

    NIIEVSKEARKR

    by a Cysteine residue linker, corresponding to C terminal amino acids 68-79 of Mouse Adipose Triglyceride Lipase

  • Positive control
    • Heart tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/32000.
IHC-P Use a concentration of 2.5 µg/ml.
WB Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
  • Tissue specificityHighest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
  • PathwayGlycerolipid metabolism; triacylglycerol degradation.
  • Involvement in diseaseNote=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
    Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells.
  • Sequence similaritiesContains 1 patatin domain.
  • Developmental stageInduced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium.
  • Cellular localizationLipid droplet. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110001C14Rik antibody
    • Adipose triglyceride lipase antibody
    • ATGL antibody
    • ATGL DESNUTRIN antibody
    • Calcium independent phospholipase A2 antibody
    • Calcium-independent phospholipase A2 antibody
    • Desnutrin antibody
    • EC 3.1.1.3 antibody
    • FP17548 antibody
    • IPLA2 zeta antibody
    • IPLA2-zeta antibody
    • PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 antibody
    • Patatin-like phospholipase domain-containing protein 2 antibody
    • PEDF R antibody
    • PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • plpl antibody
    • plpl2 antibody
    • PLPL2_HUMAN antibody
    • Pnpla2 antibody
    • Transport secretion protein 2 antibody
    • Transport secretion protein 2.2 antibody
    • Transport-secretion protein 2 antibody
    • Triglyceride hydrolase antibody
    • TTS 2.2 antibody
    • TTS2 antibody
    • TTS2.2 antibody
    • ZETA antibody
    see all

Anti-Adipose Triglyceride Lipase antibody images

  • ab85858 at 2.5 µg/ml staining Adipose Triglyceride Lipase in formalin fixed and paraffin embedded Mouse heart tissue.
  • All lanes : Anti-Adipose Triglyceride Lipase antibody (ab85858) at 1/500 dilution

    Lane 1 : Whole cell lysate prepared from undifferentiated 3T3L1
    Lane 2 : Whole cell lysate prepared from differentiated 3T3L1

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated mouse anti-goat polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview.

    See Abreview

References for Anti-Adipose Triglyceride Lipase antibody (ab85858)

ab85858 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Total protein in input 500 µg
Specification Hepa1-6 hepatoma cells
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

Submitted Apr 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Hepa1-6 hepatoma cells)
Loading amount 40 µg
Specification Hepa1-6 hepatoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Anne Jensen-Urstad

Verified customer

Submitted Apr 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3L1)
Loading amount 20 µg
Specification 3T3L1
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"