Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)

Overview

  • Product nameAnti-Adipose Triglyceride Lipase antibody [EPR3444(2)]
    See all Adipose Triglyceride Lipase primary antibodies
  • Description
    Rabbit monoclonal [EPR3444(2)] to Adipose Triglyceride Lipase
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Adipose Triglyceride Lipase aa 350-450. The exact sequence is proprietary.

  • Positive control
    • WB: 3T3-L1, A431 and A673 cell lysates, human heart, skeletal muscle and adipose tissue lysates. Mouse and rat heart tissue lysates.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionCatalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
    • Tissue specificityHighest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
    • PathwayGlycerolipid metabolism; triacylglycerol degradation.
    • Involvement in diseaseNote=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
      Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells.
    • Sequence similaritiesContains 1 patatin domain.
    • Developmental stageInduced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium.
    • Cellular localizationLipid droplet. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1110001C14Rik antibody
      • Adipose triglyceride lipase antibody
      • ATGL antibody
      • ATGL DESNUTRIN antibody
      • Calcium independent phospholipase A2 antibody
      • Calcium-independent phospholipase A2 antibody
      • Desnutrin antibody
      • EC 3.1.1.3 antibody
      • FP17548 antibody
      • IPLA2 zeta antibody
      • IPLA2-zeta antibody
      • PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 antibody
      • Patatin-like phospholipase domain-containing protein 2 antibody
      • PEDF R antibody
      • PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA antibody
      • Pigment epithelium derived factor antibody
      • Pigment epithelium-derived factor antibody
      • plpl antibody
      • plpl2 antibody
      • PLPL2_HUMAN antibody
      • Pnpla2 antibody
      • Transport secretion protein 2 antibody
      • Transport secretion protein 2.2 antibody
      • Transport-secretion protein 2 antibody
      • Triglyceride hydrolase antibody
      • TTS 2.2 antibody
      • TTS2 antibody
      • TTS2.2 antibody
      • ZETA antibody
      see all

    Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] images

    • All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/5000 dilution (purified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

      Predicted band size : 55 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (purified)

      Lane 1 : Human heart tissue lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : A673 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

      Predicted band size : 55 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (unpurified)

      Lane 1 : 3T3-L1 lysate
      Lane 2 : A431 lysate
      Lane 3 : A673 lysate
      Lane 4 : Human heart tissue lysate
      Lane 5 : Human skeletal muscle tissue lysate
      Lane 6 : Human adipose tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

    References for Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)

    This product has been referenced in:
    • Covington JD  et al. Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation. PLoS One 9:e91675 (2014). WB ; Human . Read more (PubMed: 24632837) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"