The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
FunctionATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Tissue specificityWidely expressed. Highest level in placenta, liver, muscle and kidney.
PathwayPurine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
Sequence similaritiesBelongs to the carbohydrate kinase pfkB family.