The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Detection limit for recombinant tagged ADK is approximately 10ng/ml if used as a capture antibody.
1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
FunctionATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Tissue specificityWidely expressed. Highest level in placenta, liver, muscle and kidney.
PathwayPurine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
Sequence similaritiesBelongs to the carbohydrate kinase pfkB family.