The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Widely expressed. Highest level in placenta, liver, muscle and kidney.
Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
All lanes : Anti-ADK antibody (ab88903) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg Lane 2 : ADK knockout HAP1 whole cell lysate at 40 µg Lane 3 : HepG2 whole cell lysate at 20 µg
Predicted band size: 41 kDa
Lanes 1 - 3: Merged signal (red and green). Green - ab88903 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab88903 was shown to recognize ADK in wild-type HAP1 cells as signal was lost at the expected MW in ADK knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ADK knockout samples were subjected to SDS-PAGE. Ab88903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ADK antibody (ab88903)
All lanes : Anti-ADK antibody (ab88903) at 1 µg/ml
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate Lane 2 : Human placenta tissue lysate - total protein (ab29745) Lane 3 : Human liver tissue lysate - total protein (ab29889) Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Martínez-Vega R et al. Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism. FASEB J29:418-32 (2015).
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