Overview

  • Product name
  • Description
    Rabbit polyclonal to ADK
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Chinese hamster
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human ADK.

  • Positive control
    • This antibody gave a positive signal in the following lysates: RAW 264.7 Whole Cell Lysate; Human Placenta Tissue Lysate; Human Liver Tissue Lysate; HepG2 Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

Images

  • All lanes : Anti-ADK antibody (ab88903) at 1 µg/ml

    Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 39,41 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

References

This product has been referenced in:
  • Martínez-Vega R  et al. Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism. FASEB J 29:418-32 (2015). Read more (PubMed: 25384423) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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