Overview

  • Product name
    Anti-Adrenomedullin antibody
    See all Adrenomedullin primary antibodies
  • Description
    Rabbit polyclonal to Adrenomedullin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, PigDoes not react with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Adrenomedullin.

    (Peptide available as ab69116.)

  • Positive control
    • Thia antibody gave a positive signal in the following Human Lysates: Breast Tissue MCF7 Whole Cell DU 145 Whole Cell MDA-MB-361 Whole Cell This antibody gave a positive result in IHC in the following FFPE tissue: Human placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab69117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Adrenomedullin peptide (ab69116).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
  • Tissue specificity
    Highest levels found in pheochromocytoma and adrenal medulla. Also found in lung, ventricle and kidney tissues.
  • Sequence similarities
    Belongs to the adrenomedullin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adm antibody
    • ADM precursor antibody
    • ADML_HUMAN antibody
    • AM antibody
    • PAMP antibody
    • Preproadrenomedullin antibody
    • Proadrenomedullin N-20 terminal peptide antibody
    • Proadrenomedullin N20 terminal peptide antibody
    • ProAM N terminal 20 peptide antibody
    • ProAM N-terminal 20 peptide antibody
    • ProAM N20 antibody
    • ProAM-N20 antibody
    • ProAMN20 antibody
    see all

Images

  • All lanes : Anti-Adrenomedullin antibody (ab69117) at 1 µg/ml

    Lane 1 : Human breast tissue lysate - total protein (ab30090)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 4 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 170 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of Adrenomedullin staining in Human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69117, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Albertin G  et al. Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells. Regul Pept : (2011). WB ; Human . Read more (PubMed: 21871928) »

See 1 Publication for this product

Customer reviews and Q&As

We have not carried out a peptide blocking study for this product. This has been characterised using WB against MCF7, DU145 and MDA MB 361, and IHC against Human plancenta.


We guarantee the antibody to be specific for adrenomedullin for 1...

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Application
Western blot
Loading amount
30000 cells
Gel Running Conditions
Reduced Denaturing (11)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblast (MEF))
Specification
Mouse embryonic fibroblast (MEF)
Treatment
0.5% oxygen for 6hrs
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 05 2013

Application
Western blot
Loading amount
30000 cells
Gel Running Conditions
Reduced Denaturing (11%)
Sample
Human Cell lysate - whole cell (MCF-7 cells (human breast adenocarcinoma cell line)
Specification
MCF-7 cells (human breast adenocarcinoma cell line
Treatment
0.5% oxygen for 16hrs
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 05 2013

I can confirm that this antibody has been tested for both applications Western blot (WB) and IHC on formalin-fixed paraffin-embedded tissue sections (IHC-P) using human samples. The cross-reactivity with other species has not been determined yet, thoug...

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