Anti-Adrenomedullin antibody [HTA171/E8] (ab18092)


  • Product name
    Anti-Adrenomedullin antibody [HTA171/E8]
    See all Adrenomedullin primary antibodies
  • Description
    Mouse monoclonal [HTA171/E8] to Adrenomedullin
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Chemically synthesized full length adrenomedullin.



Our Abpromise guarantee covers the use of ab18092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC-P 1/50. PubMed: 18445660
IHC-Fr Use at an assay dependent concentration.


  • Function
    AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
  • Tissue specificity
    Highest levels found in pheochromocytoma and adrenal medulla. Also found in lung, ventricle and kidney tissues.
  • Sequence similarities
    Belongs to the adrenomedullin family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Adm antibody
    • ADM precursor antibody
    • ADML_HUMAN antibody
    • AM antibody
    • PAMP antibody
    • Preproadrenomedullin antibody
    • Proadrenomedullin N-20 terminal peptide antibody
    • Proadrenomedullin N20 terminal peptide antibody
    • ProAM N terminal 20 peptide antibody
    • ProAM N-terminal 20 peptide antibody
    • ProAM N20 antibody
    • ProAM-N20 antibody
    • ProAMN20 antibody
    see all


  • Overlay histogram showing PC12 cells stained with ab18092 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18092, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Kraus DM  et al. Cigarette Smoke-Induced Placental Adrenomedullin Expression and Trophoblast Cell Invasion. Reprod Sci N/A:N/A (2013). Read more (PubMed: 23653390) »
  • Petalidis LP  et al. Improved grading and survival prediction of human astrocytic brain tumors by artificial neural network analysis of gene expression microarray data. Mol Cancer Ther : (2008). IHC-P ; Human . Read more (PubMed: 18445660) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us for technical support with ab18092. This antibody has not been tested in western blotting, only in immunohistochemistry, and your protocol looks very good so this suggest to me that the antibody may only recognise the native...

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