Recombinant Anti-GPR182 antibody [EPR14327] (ab182555)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14327] to GPR182
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-GPR182 antibody [EPR14327]
See all GPR182 primary antibodies -
Description
Rabbit monoclonal [EPR14327] to GPR182 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human skeletal muscle and Human fetal heart lysates; HT-1376 and U-87 MG cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14327 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
-
Function
Orphan receptor. -
Tissue specificity
Highly expressed in heart, skeletal muscle, immune system, adrenal gland and liver. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 11318 Human
- Entrez Gene: 11536 Mouse
- Omim: 605307 Human
- SwissProt: O15218 Human
- SwissProt: P43142 Mouse
- Unigene: 483909 Human
- Unigene: 2857 Mouse
-
Alternative names
- 7TMR antibody
- ADMR antibody
- Adrenomedullin L1 receptor antibody
see all
Images
-
All lanes : Anti-GPR182 antibody [EPR14327] (ab182555) at 1/5000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa -
All lanes : Anti-GPR182 antibody [EPR14327] (ab182555) at 1/5000 dilution
Lane 1 : HT-1376 cell lysate
Lane 2 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab182555 has been referenced in 1 publication.
- Xia J et al. A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33785593