Overview

  • Product name
  • Description
    Rabbit polyclonal to ADRM1
  • Tested applications
    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 357-407 of Human ADRM1 (NP_008933.2).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab91567 was affinity purified using an epitope specific to ADRM1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 5-15 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.
  • Sequence similarities
    Belongs to the ADRM1 family.
    Contains 1 PH domain.
  • Domain
    The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 110 kDa cell membrane glycoprotein antibody
    • adhesion regulating molecule 1 antibody
    • Adhesion-regulating molecule 1 antibody
    • ADRM 1 antibody
    • Adrm1 antibody
    • ADRM1_HUMAN antibody
    • ARM 1 antibody
    • ARM-1 antibody
    • ARM1 antibody
    • Gp110 antibody
    • hRpn13 antibody
    • M(r) 110,000 surface antigen antibody
    • Proteasomal ubiquitin receptor ADRM1 antibody
    • proteasome regulatory particle non ATPase 13 antibody
    • Proteasome regulatory particle non-ATPase 13 antibody
    • Regulatory particle non ATPase 13 antibody
    • Rpn13 antibody
    • Rpn13 homolog antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ADRM1 with ab91567 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ADRM1 antibody (ab91567) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 42 kDa


    Exposure time : 3 minutes
  • Detection of ADRM1 by Western Blot of Immunprecipitate.
    ab91567, at 1 µg/ml, staining ADRM1 in HeLa whole cell lysate immunoprecipitated using ab91567 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:
  • Huang Y & Ratovitski EA Phosphorylated TP63 induces transcription of RPN13, leading to NOS2 protein degradation. J Biol Chem 285:41422-31 (2010). WB ; Human . Read more (PubMed: 20959455) »
  • Huang Y & Ratovitski EA Phospho-?Np63a/Rpn13-dependent regulation of LKB1 degradation modulates autophagy in cancer cells. Aging (Albany NY) 2:959-68 (2010). Read more (PubMed: 21191146) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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