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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab91567 was affinity purified using an epitope specific to ADRM1 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 5-15 µg/mg of lysate.
1/2000 - 1/10000. Predicted molecular weight: 42 kDa.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity.
Belongs to the ADRM1 family.
Contains 1 PH domain.
The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit.
Information by UniProt
110 kDa cell membrane glycoprotein antibody
adhesion regulating molecule 1 antibody
Adhesion-regulating molecule 1 antibody
Anti-ADRM1 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADRM1 antibody (ab91567)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ADRM1 with ab91567 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - ADRM1 antibody (ab91567)
All lanes : Anti-ADRM1 antibody (ab91567) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 42 kDa Exposure time : 3 minutes
Immunoprecipitation - ADRM1 antibody (ab91567)
Detection of ADRM1 by Western Blot of Immunprecipitate.
ab91567, at 1 µg/ml, staining ADRM1 in HeLa whole cell lysate immunoprecipitated using ab91567 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-ADRM1 antibody (ab91567)
This product has been referenced in:
Huang Y & Ratovitski EA
Phosphorylated TP63 induces transcription of RPN13, leading to NOS2 protein degradation. J Biol Chem 285:41422-31 (2010).
Read more (PubMed: 20959455) »
Huang Y & Ratovitski EA
Phospho-?Np63a/Rpn13-dependent regulation of LKB1 degradation modulates autophagy in cancer cells. Aging (Albany NY) 2:959-68 (2010).
Read more (PubMed: 21191146) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"