Recombinant Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11450(B)] to ADRM1/ARM-1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ADRM1/ARM-1 antibody [EPR11450(B)]
See all ADRM1/ARM-1 primary antibodies -
Description
Rabbit monoclonal [EPR11450(B)] to ADRM1/ARM-1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 350 to the C-terminus.
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Positive control
- WB: HEK293T, K562, Raji, HeLa, Raw264.7 and NIH 3T3 cell lysates. IHC-P: Human testis tissue, Human thyroid gland carcinoma tissue. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11450(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity. -
Sequence similarities
Belongs to the ADRM1 family.
Contains 1 PH domain. -
Domain
The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11047 Human
- Entrez Gene: 56436 Mouse
- Omim: 610650 Human
- SwissProt: Q16186 Human
- SwissProt: Q9JKV1 Mouse
- Unigene: 90107 Human
- Unigene: 379082 Mouse
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Alternative names
- 110 kDa cell membrane glycoprotein antibody
- adhesion regulating molecule 1 antibody
- Adhesion-regulating molecule 1 antibody
see all
Images
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All lanes : Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ADRM1 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaLanes 1-4: Merged signal (red and green). Green - ab157218 observed at 42 kDa. Red - loading control ab8245 observed at 36 kDa.
ab157218 Anti-ADRM1/ARM-1 antibody [EPR11450(B)] was shown to specifically react with ADRM1/ARM-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266765 (knockout cell lysate ab257816) was used. Wild-type and ADRM1/ARM-1 knockout samples were subjected to SDS-PAGE. ab157218 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin embedded Human testis tissue labeling ADRM1/ARM-1 with ab157218 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ADRM1/ARM-1 antibody [EPR11450(B)] (ab157218) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa -
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling ADRM1/ARM-1 with ab157218 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized Hela cells using ab157218 at 1/10 (red) or a rabbit IgG negative (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab157218 has been referenced in 3 publications.
- Lu X et al. Structure-guided bifunctional molecules hit a DEUBAD-lacking hRpn13 species upregulated in multiple myeloma. Nat Commun 12:7318 (2021). PubMed: 34916494
- Feng Q et al. Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy. Front Immunol 9:1881 (2018). PubMed: 30177931
- Huang ZN et al. Adhesion Regulating Molecule 1 Mediates HAP40 Overexpression-Induced Mitochondrial Defects. Int J Biol Sci 13:1420-1437 (2017). PubMed: 29209146