Overview

  • Product name
  • Description
    Rabbit polyclonal to Adropin
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SADVDSLSES SPNSSPGPCP EKAPPPQKPS HEGSYLLQ, corresponding to amino acids of Human Adropin.

  • Positive control
    • Human smooth muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Involved in the regulation of glucose homestasis and lipid metabolism.
  • Tissue specificity
    Expressed in liver and brain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adropin antibody
    • C9orf165 antibody
    • Energy homeostasis associated protein antibody
    • Energy homeostasis-associated protein antibody
    • ENHO antibody
    • ENHO_HUMAN antibody
    • PRO830 antibody
    • UNQ470 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, plasma membrane, cytoplasm and focal adhesions. Recommended concentration of ab122800 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122800, at 1/200 dilution, staining Adropin in Paraffin-embedded Human smooth muscle tissue by Immunohistochemistry

References

This product has been referenced in:
  • Kuloglu T & Aydin S Immunohistochemical expressions of adropin and inducible nitric oxide synthase in renal tissues of rats with streptozotocin-induced experimental diabetes. Biotech Histochem N/A:N/A (2013). Read more (PubMed: 23957703) »
  • Aydin S  et al. Expression of adropin in rat brain, cerebellum, kidneys, heart, liver, and pancreas in streptozotocin-induced diabetes. Mol Cell Biochem 380:73-81 (2013). Read more (PubMed: 23620340) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain homogenate)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
brain homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eduardo Candelario-Jalil

Verified customer

Submitted May 23 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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