The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately <20, 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionComponent of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.
Tissue specificityPredominantly expressed in skeletal muscle and heart, as well as in several hematopoietic cell lines and solid tumors.
PathwayPurine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Sequence similaritiesBelongs to the adenylosuccinate synthetase family.