Overview

  • Product name
  • Description
    Rabbit polyclonal to AEBP2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Pig, Monkey, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human AEBP2.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Fetal Liver: Mouse Liver; Mouse Brain; Mouse Heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab107892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • Function
    DNA-binding transcriptional repressor. May interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
  • Sequence similarities
    Belongs to the AEBP2/jing C2H2-type zinc-finger family.
    Contains 3 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adipocyte enhancer binding protein 2 antibody
    • Adipocyte enhancer-binding protein 2 antibody
    • AE (adipocyte enhancer) binding protein 2 antibody
    • AE binding protein 2 antibody
    • AE-binding protein 2 antibody
    • AEBP 2 antibody
    • AEBP2 antibody
    • AEBP2_HUMAN antibody
    • MGC17922 antibody
    • Zinc finger protein AEBP2 antibody
    see all

Images

  • All lanes : Anti-AEBP2 antibody (ab107892) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 35 kDa,68 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References

This product has been referenced in:
  • Nguyen K  et al. Multiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency. MBio 8:N/A (2017). WB ; Human . Read more (PubMed: 28246360) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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