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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Purity Protein G purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight for isoform 3: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Relevance Belongs to the glycosyltransferase 61 family.
Cellular localization Secreted
AER61 glycosyltransferase antibody
EGF domain-specific O-linked N-acetylglucosamine transferase antibody
Anti-AER61 antibody images
Western blot - AER61 antibody (ab69389)
All lanes : Anti-AER61 antibody (ab69389) at 1/500 dilution Lane 1 : AER61 transfected 293T cell lysate Lane 2 : Non transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Predicted band size : 52 kDa Observed band size : 50 kDa ( why is the actual band size different from the predicted?)
References for Anti-AER61 antibody (ab69389)
This product has been referenced in:
Tashima Y & Stanley P
Antibodies that detect O-linked ß-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem 289:11132-42 (2014).
Read more (PubMed: 24573683) »
et al. The EGF repeat-specific O-GlcNAc-transferase Eogt interacts with notch signaling and pyrimidine metabolism pathways in Drosophila. PLoS One 8:e62835 (2013).
Read more (PubMed: 23671640) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"