Overview

  • Product name
  • Description
    Goat polyclonal to AF10
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Chicken, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide (C-

    RLEDTTARFTNAN

    ) corresponding to internal sequence amino acids 281-293 of Human AF10 (NP_004632.1).

  • Positive control
    • Rat testis lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab101719 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 109 kDa.

Target

  • Function
    Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA.
  • Tissue specificity
    Expressed abundantly in testis.
  • Involvement in disease
    A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with KMT2A/MLL1. The result is a rogue activator protein.
    A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM.
  • Sequence similarities
    Contains 1 C2HC pre-PHD-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF 10 antibody
    • AF10 antibody
    • AF10_HUMAN antibody
    • ALL 1 fused gene from chromosome 10 antibody
    • ALL1 fused gene from chromosome 10 antibody
    • ALL1-fused gene from chromosome 10 protein antibody
    • DKFZp686E10210 antibody
    • MGC75086 antibody
    • MLLT 10 antibody
    • MLLT10 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10 antibody
    • Myeloid/lymphoid or mixed lineage leukemia translocated to 10 antibody
    • OTTHUMP00000045273 antibody
    • Protein AF 10 antibody
    • Protein AF-10 antibody
    • Protein AF10 antibody
    • Type I AF10 protein antibody
    see all

Images

  • Anti-AF10 antibody (ab101719) at 1 µg/ml + Rat testis lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 109 kDa
    Observed band size : ~140,~60 kDa (why is the actual band size different from the predicted?)

References

ab101719 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab101719.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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