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Rabbit polyclonal to AF10 - C-terminal
Synthetic peptide, corresponding to a region within C terminal amino acids 965-1027 of Human AF10 (UniProt ID: P55197).
A431, H1299, HeLa, HepG2 whole cell lysate; SCC xenograft
Keep as a concentrated solution.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 109 kDa.
1/100 - 1/1000. Suggested antigen retrieval
using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA.
Expressed abundantly in testis.
Involvement in disease
A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with KMT2A/MLL1. The result is a rogue activator protein.
A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM.
Contains 1 C2HC pre-PHD-type zinc finger.
Contains 2 PHD-type zinc fingers.
Information by UniProt
AF 10 antibody
Western blot - Anti-AF10 antibody - C-terminal (ab155205)
Anti-AF10 antibody - C-terminal (ab155205) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 109 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AF10 antibody - C-terminal (ab155205)
Immunohistochemical analysis of paraffin-embedded SCC xenograft tissue labeling AF10 with ab155205 at 1/100 dilution.
has not yet been referenced specifically in any publications.
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