Overview

  • Product name
    Anti-AF10 antibody [HAF10 10D3/8]
    See all AF10 primary antibodies
  • Description
    Mouse monoclonal [HAF10 10D3/8] to AF10
  • Tested applications
    Suitable for: WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    RLSDKTGPVAQEKS

    , corresponding to amino acids 1055-1069 of Human AF10

Properties

Applications

Our Abpromise guarantee covers the use of ab16969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 110 kDa.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • Function
    Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA.
  • Tissue specificity
    Expressed abundantly in testis.
  • Involvement in disease
    A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with KMT2A/MLL1. The result is a rogue activator protein.
    A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM.
  • Sequence similarities
    Contains 1 C2HC pre-PHD-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF 10 antibody
    • AF10 antibody
    • AF10_HUMAN antibody
    • ALL 1 fused gene from chromosome 10 antibody
    • ALL1 fused gene from chromosome 10 antibody
    • ALL1-fused gene from chromosome 10 protein antibody
    • DKFZp686E10210 antibody
    • MGC75086 antibody
    • MLLT 10 antibody
    • MLLT10 antibody
    • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10 antibody
    • Myeloid/lymphoid or mixed lineage leukemia translocated to 10 antibody
    • OTTHUMP00000045273 antibody
    • Protein AF 10 antibody
    • Protein AF-10 antibody
    • Protein AF10 antibody
    • Type I AF10 protein antibody
    see all

References for Anti-AF10 antibody [HAF10 10D3/8] (ab16969)

This product has been referenced in:
  • Bitoun E  et al. The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling. Hum Mol Genet 16:92-106 (2007). WB ; Mouse . Read more (PubMed: 17135274) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3-L1 differentiated adipocytes)
Loading amount
50 µg
Specification
3T3-L1 differentiated adipocytes
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Ainhoa Ceballos

Verified customer

Submitted Apr 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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