Overview

  • Product name
  • Description
    Rabbit polyclonal to AF9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 281-532 of Human AF9 (Uniprot ID P42568).

  • Positive control
    • THP1 whole cell lysate. HeLa cells. D54 xenograft tissue.
  • General notes
    Store as a concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 63 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Involvement in disease
    Note=A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities
    Contains 1 YEATS domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF 9 antibody
    • AF9_HUMAN antibody
    • ALL1 fused gene from chromosome 9 protein antibody
    • ALL1-fused gene from chromosome 9 protein antibody
    • FLJ2035 antibody
    • MLLT3 antibody
    • Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein antibody
    • Protein AF-9 antibody
    • YEATS 3 antibody
    • YEATS domain containing protein 3 antibody
    • YEATS domain-containing protein 3 antibody
    • YEATS3 antibody
    see all

Images

  • Anti-AF9 antibody (ab154492) at 1/1000 dilution + THP1 whole cell lysate at 30 µg

    Predicted band size: 63 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling AF9 using ab154492 at 1/500 dilution. Right panel costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded D54 xenograft , labeling AF9 using ab154492 at 1/750 dilution.

References

ab154492 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (primary bone marrow derived macrophages)
Specification
primary bone marrow derived macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Sep 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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