Anti-AF9 antibody (ab154492)
Key features and details
- Rabbit polyclonal to AF9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AF9 antibody -
Description
Rabbit polyclonal to AF9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A recombinant fragment corresponding to a region within amino acids 281-532 of Human AF9 (Uniprot ID P42568).
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Positive control
- THP1 whole cell lysate. HeLa cells. D54 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 63 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 63 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Involvement in disease
Note=A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein. -
Sequence similarities
Contains 1 YEATS domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4300 Human
- Entrez Gene: 70122 Mouse
- Omim: 159558 Human
- SwissProt: P42568 Human
- SwissProt: A2AM29 Mouse
- Unigene: 591085 Human
- Unigene: 288898 Mouse
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Alternative names
- AF 9 antibody
- AF9_HUMAN antibody
- ALL1 fused gene from chromosome 9 protein antibody
see all
Images
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Anti-AF9 antibody (ab154492) at 1/1000 dilution + THP1 whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling AF9 using ab154492 at 1/500 dilution. Right panel costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded D54 xenograft , labeling AF9 using ab154492 at 1/750 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154492 has been referenced in 3 publications.
- Zhai J et al. Primate gastrulation and early organogenesis at single-cell resolution. Nature 612:732-738 (2022). PubMed: 36517595
- Liu B et al. Yeats4 drives ILC lineage commitment via activation of Lmo4 transcription. J Exp Med 216:2653-2668 (2019). PubMed: 31434684
- Kuzmina A et al. Super elongation complex promotes early HIV transcription and its function is modulated by P-TEFb. Transcription 8:133-149 (2017). PubMed: 28340332