Overview

  • Product name
  • Description
    Rabbit polyclonal to AFAF
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: SCESQYSVNP ELATMSYFHP SEGVSDTSFS KSAESSTFLG TTSSDMRRSG TRTSESKIMT DIISIGSDNE MHENDESVTR, corresponding to amino acids 215-294 of Human AFAF

  • Positive control
    • Human testis tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Involved in acrosome biogenesis.
  • Tissue specificity
    Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > acrosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrosome formation-associated factor antibody
    • Afaf antibody
    • AFAF_HUMAN antibody
    • C9orf11 antibody
    • chromosome 9 open reading frame 11 antibody
    • equatorin antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121461 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121461 at a 1/300 dilution staining AFAF in Human testis tissue (paraffin embedded) by immunohistochemistry.

References

ab121461 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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