Overview

  • Product nameAnti-AFAF antibody
    See all AFAF primary antibodies
  • Description
    Rabbit polyclonal to AFAF
  • Specificityab121470 shows specific reactivity against Isoform 1 of Human AFAF.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 35-182 of Human AFAF.

  • Positive control
    • Human testis tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in acrosome biogenesis.
  • Tissue specificityIsoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.
  • Cellular localizationCytoplasmic vesicle > secretory vesicle > acrosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrosome formation-associated factor antibody
    • Afaf antibody
    • AFAF_HUMAN antibody
    • C9orf11 antibody
    • chromosome 9 open reading frame 11 antibody
    • equatorin antibody
    see all

Anti-AFAF antibody images

  • Lane 1 :
    Lanes 2 - 3 : Anti-AFAF antibody (ab121470)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing AFAF with a C-terminal myc-DDK tag (~3.1kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling AFAF with ab121470. Staining shows strong cytoplasmic positivity in spermatids.

References for Anti-AFAF antibody (ab121470)

ab121470 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"