Overview

  • Product name
  • Description
    Rabbit polyclonal to AFAF
  • Specificity
    ab121470 shows specific reactivity against Isoform 1 of Human AFAF.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 35-182 of Human AFAF.

  • Positive control
    • Human testis tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in acrosome biogenesis.
  • Tissue specificity
    Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle > acrosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acrosome formation-associated factor antibody
    • Afaf antibody
    • AFAF_HUMAN antibody
    • C9orf11 antibody
    • chromosome 9 open reading frame 11 antibody
    • equatorin antibody
    see all

Images

  • Lane 1 :
    Lanes 2 - 3 : Anti-AFAF antibody (ab121470)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing AFAF with a C-terminal myc-DDK tag (~3.1kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling AFAF with ab121470. Staining shows strong cytoplasmic positivity in spermatids.

References

ab121470 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121470.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up