• Product name
  • Description
    Rabbit polyclonal to AFF2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 1224-1273 (AMGNCNNGPV TIPQRIHHMA ASHVNITSNV LRGYEHWDMA DKLTRENKEF) of Human AFF2 (NP_002016).

  • Positive control
    • Human fetal lung lysate.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab85450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 145 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    RNA-binding protein. Might be involved in alternative splicing regulation through an interaction with G-quartet RNA structure.
  • Tissue specificity
    Brain (most abundant in hippocampus and amygdala), placenta and lung.
  • Involvement in disease
    Mental retardation, X-linked, associated with fragile site FRAXE
  • Sequence similarities
    Belongs to the AF4 family.
  • Cellular localization
    Nucleus speckle. When splicing is inhibited, accumulates in enlarged speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF4/FMR2 family member 2 antibody
    • AF4/FMR2 family, member 2 antibody
    • AFF2 antibody
    • AFF2_HUMAN antibody
    • FMR2 antibody
    • FMR2P antibody
    • Fragile X E mental retardation syndrome protein antibody
    • fragile X mental retardation 2 antibody
    • Fragile X mental retardation 2 protein antibody
    • fragile X mental retardation gene associated with FRAXE antibody
    • FRAXE antibody
    • mild or borderline mental retardation antibody
    • MRX2 antibody
    • OTTHUMP00000024204 antibody
    • OX19 antibody
    • Protein FMR-2 antibody
    • Protein Ox19 antibody
    see all

Anti-AFF2 antibody images

  • Anti-AFF2 antibody (ab85450) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 145 kDa
    Observed band size : ~160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-AFF2 antibody (ab85450)

ab85450 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. Based on the information you have told me, it appears that there could be an issue with the antibody, since it is not recognizing the band at 160kDa. I would be happy to either send you a replacement antibody or to pro...

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Thank you for your reply. I can send you 1 of the other AFF2 antibodies as a free of charge replacement. If you let me know which of these antibodies that you would like to receive, I will be happy to have it shipped as soon as it comes back in...

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Thank you for your reply. I am sorry about the problems you are having. We currently have 3 AFF2 antibodies in our catalogue and unfortunately all of them are out of stock and have a estimated delivery time of 1-2 weeks. I would be happy to send any of...

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Thank you for contacting Abcam. From what I have been reading on AFF2, it appears that there are multiply variants of AFF2 and that could explain why you are detecting multiply different bands. It could be that the different antibodies are reco...

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